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验证数据展示

  • WB analysis of SH-SY5Y using 17306-1-AP

    WB analysis of SH-SY5Y using 17306-1-AP

    WB result of NLN antibody (17306-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NLN transfected SH-SY5Y cells.

  • WB analysis using 17306-1-AP

    WB analysis using 17306-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17306-1-AP (NLN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

NLN Polyclonal antibody

NLN Polyclonal Antibody for WB, ELISA
Cat No. 17306-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

AGTBP, Angiotensin binding protein, Angiotensin-binding protein, EC:3.4.24.16, KIAA1226

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSH-SY5Y cells, mouse heart tissue, rat heart tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17306-1-AP targets NLN in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11193

Product name: Recombinant human NLN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 280-607 aa of BC004985

Sequence: LQQLLPLRTKVAKLLGYSTHADFVLEMNTAKSTSRVTAFLDDLSQKLKPLGEAEREFILNLKKKECKDRGFEYDGKINAWDLYYYMTQTEELKYSIDQEFLKEYFPIEVVTEGLLNTYQELLGLSFEQMTDAHVWNKSVTLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMAVAALVVNFSQPVAGRPSLLRHDEVRTYFHEFGHVMHQICAQVSFFFPPVNLPISFFRKLLDCCQVSAPNRFFQKLNVFKNFIGLLQNQNFPYPVLFHNIFLLGGSSTSERCLLNNSLWKKNKTICKERLQILCFFLLIL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neurolysin (metallopeptidase M3 family)
别名 AGTBP, Angiotensin binding protein, Angiotensin-binding protein, EC:3.4.24.16, KIAA1226
计算分子量 607 aa, 70 kDa
观测分子量70-80 kDa
GenBank蛋白编号BC004985
基因名称 NLN
Gene ID (NCBI) 57486
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BYT8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NLN(Neurolysin, mitochondrial) is also named as AGTBP, KIAA1226 and belongs to the peptidase M3 family. It is an enzyme involved in the metabolic inactivation of bioactive peptides and functions as a monomer of molecular mass 78 kDa. It also can be dected the molecular weight between 70 kDa and 85 kDa according to the reports(PMID:10095765, 11092934). The full length protein has a transit peptide with 37 amino acids.

实验方案

Product Specific Protocols
WB protocol for NLN antibody 17306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols