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验证数据展示

  • WB analysis of mouse ovary using 14763-1-AP

    WB analysis of mouse ovary using 14763-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14763-1-AP (NLN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 14763-1-AP

    IHC staining of mouse testis using 14763-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14763-1-AP (NLN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NLN Polyclonal antibody

NLN Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14763-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

AGTBP, Angiotensin binding protein, KIAA1226, MEP, Microsomal endopeptidase, Mitochondrial oligopeptidase M, Neurolysin, mitochondrial, Neurotensin endopeptidase, NLN

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse ovary tissue, HepG2 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14763-1-AP targets NLN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NLN fusion protein Ag6524 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neurolysin (metallopeptidase M3 family)
别名 AGTBP, Angiotensin binding protein, KIAA1226, MEP, Microsomal endopeptidase, Mitochondrial oligopeptidase M, Neurolysin, mitochondrial, Neurotensin endopeptidase, NLN
计算分子量 81 kDa
观测分子量 75 kDa
GenBank蛋白编号BC001644
基因名称 NLN
Gene ID (NCBI) 57486
RRIDAB_2151973
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BYT8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NLN(Neurolysin, mitochondrial) is also named as AGTBP, KIAA1226 and belongs to the peptidase M3 family. It is an enzyme involved in the metabolic inactivation of bioactive peptides and functions as a monomer of molecular mass 78 kDa. It also can be dected the molecular weight between 70 kDa and 85 kDa according to the reports (PMID:10095765, 11092934). The full length protein has a transit peptide with 37 amino acids.

实验方案

Product Specific Protocols
WB protocol for NLN antibody 14763-1-APDownload protocol
IHC protocol for NLN antibody 14763-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols