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验证数据展示

  • WB analysis of mouse brain using 66964-1-Ig (same clone as 66964-1-PBS)

    WB analysis of mouse brain using 66964-1-Ig (same clone as 66964-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66964-1-PBS in a different storage buffer formulation.

  • WB analysis of rat brain using 66964-1-Ig (same clone as 66964-1-PBS)

    WB analysis of rat brain using 66964-1-Ig (same clone as 66964-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66964-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66964-1-Ig (same clone as 66964-1-PBS)

    IHC staining of mouse brain using 66964-1-Ig (same clone as 66964-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66964-1-Ig (NLGN1 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66964-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NLGN1 antibody included

NLGN1 Monoclonal antibody, PBS Only

NLGN1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66964-1-PBS

产品说明书

CloneNo. 2D1A12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, Indirect ELISA

别名

NLG1, KIAA1070, neuroligin 1, Neuroligin-1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NLGN1 Ready-To-Use IHC Kit. NLGN1 primary antibody included.

产品信息

66964-1-PBS targets NLGN1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag6356

Product name: Recombinant human NLGN1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 40-406 aa of BC032555

Sequence: AGHVLSQKLDDVDPLVATNFGKIRGIKKELNNEILGPVIQFLGVPYAAPPTGERRFQPPEPPSPWSDIRNATQFAPVCPQNIIDGRLPEVMLPVWFTNNLDVVSSYVQDQSEDCLYLNIYVPTEDDIRDSGGPKPVMVYIHGGSYMEGTGNLYDGSVLASYGNVIVITVNYRLGVLGFLSTGDQAAKGNYGLLDLIQALRWTSENIGFFGGDPLRITVFGSGAGGSCVNLLTLSHYSEGNRWSNSTKGLFQRAIAQSGTALSSWAVSFQPAKYARMLATKVGCNVSDTVELVECLQKKPYKELVDQDIQPARYHIAFGPVIDGDVIPDDPQILMEQGEFLNYDIMLGVNQGEGLKFVENIVDSDDGI

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 neuroligin 1
别名 NLG1, KIAA1070, neuroligin 1, Neuroligin-1
计算分子量 840 aa, 94 kDa
观测分子量 116 kDa
GenBank蛋白编号BC032555
基因名称 NLGN1
Gene ID (NCBI) 22871
RRIDAB_2882287
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ8N2Q7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NLGN1, also named KIAA1070, belongs to the type-B carboxylesterase/lipase family. Neuroligin1 (NLGN1) is a main component of the excitatory glutamatergic synapses complex and plays a role in synapse assembly and function (PMID: 34340665). NLGN1 has 2 isoforms with the MV of 92 and 96 kDa. Sometimes higher molecular weight of around 116 kDa can also be observed, which is a glycosylated mature form of NLGN1 (PMID: 28841651).

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