验证数据展示
经过测试的应用
| Positive WB detected in | human heart tissue |
| Positive IHC detected in | human brain tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse embryo tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13921-1-AP targets NKX2-5 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag4930 Product name: Recombinant human NKX2-5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-324 aa of BC025711 Sequence: MFPSPALTPTPFSVKDILNLEQQQRSLAAAGELSARLEATLAPSSCMLAAFKPEAYAGPEAAAPGLPELRAELGRAPSPAKCASAFPAAPAFYPRAYSDPDPAKDPRAEKKELCALQKAVELEKTEADNAERPRARRRRKPRVLFSQAQVYELERRFKQQRYLSAPERDQLASVLKLTSTQVKIWFQNRRYKCKRQRQDQTLELVGLPPPPPPPARRIAVPVLVRDGKPCLGDSAPYAPAYGVGLNPYGYNAYPAYPGYGGAACSPGYSCTAAYPAGPSPAQPATAAANNNFVNFGVGDLNAVQSPGIPQSNSGVSTLHGIRAW 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NK2 transcription factor related, locus 5 (Drosophila) |
| 别名 | NKX25, Homeobox protein Nkx-2.5, Homeobox protein Nkx 2.5, Homeobox protein NK-2 homolog E, CSX |
| 计算分子量 | 324 aa, 35 kDa |
| 观测分子量 | 30-42 kda |
| GenBank蛋白编号 | BC025711 |
| 基因名称 | NKX2-5 |
| Gene ID (NCBI) | 1482 |
| RRID | AB_1851479 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P52952 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development [PMID: 7665173]. NKX2-5 encoded by homeobox-containing genes that implicated in commitment to and/or differentiation of the myocardial lineage, and acts as a transcriptional activator of ANF in cooperation with GATA4. During embryonic development, NKX2-5 plays pivotal roles in specifying cardiac progenitors, cardiac morphogenesis, cardiomyocyte differentiation and conduction system development [PMID: 22824467].
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NKX2-5 antibody 13921-1-AP | Download protocol |
| IHC protocol for NKX2-5 antibody 13921-1-AP | Download protocol |
| WB protocol for NKX2-5 antibody 13921-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
iScience A dual SHOX2:GFP; MYH6:mCherry knockin hESC reporter line for derivation of human SAN-like cells. | ||
Am J Physiol Heart Circ Physiol Homocysteine downregulates cardiac homeobox transcription factor NKX2.5 via IGFBP5. | ||
J Appl Toxicol The effect of maternal exposure to di-(2-ethylhexyl)-phthalate on fetal cardiac development in mice. | ||
Mol Med Rep H3K9me2 regulates early transcription factors to promote mesenchymal stem‑cell differentiation into cardiomyocytes. | ||
Int J Clin Exp Pathol SDF-1α promotes repair of myocardial ischemic necrosis zones in rats. |
