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- KD/KO Validated
NKX2-5 Polyclonal antibody
NKX2-5 Polyclonal Antibody for IF-P, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human heart tissue |
| Positive IHC detected in | human brain tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse embryo tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13921-1-AP targets NKX2-5 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IF-P, IHC, WB, ELISA |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Immunogen | NKX2-5 fusion protein Ag4930 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | NK2 transcription factor related, locus 5 (Drosophila) |
| Synonyms | Cardiac specific homeobox, CHNG5, CSX, CSX1, Homeobox protein CSX, Homeobox protein Nkx 2.5, NKX2 5, NKX2.5, NKX2-5, NKX2E, NKX4 1 |
| Calculated Molecular Weight | 324 aa, 35 kDa |
| Observed Molecular Weight | 30-42 kda |
| GenBank Accession Number | BC025711 |
| Gene Symbol | NKX2-5 |
| Gene ID (NCBI) | 1482 |
| RRID | AB_1851479 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P52952 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development [PMID: 7665173]. NKX2-5 encoded by homeobox-containing genes that implicated in commitment to and/or differentiation of the myocardial lineage, and acts as a transcriptional activator of ANF in cooperation with GATA4. During embryonic development, NKX2-5 plays pivotal roles in specifying cardiac progenitors, cardiac morphogenesis, cardiomyocyte differentiation and conduction system development [PMID: 22824467].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NKX2-5 antibody 13921-1-AP | Download protocol |
| IHC protocol for NKX2-5 antibody 13921-1-AP | Download protocol |
| IF protocol for NKX2-5 antibody 13921-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
iScience A dual SHOX2:GFP; MYH6:mCherry knockin hESC reporter line for derivation of human SAN-like cells. | ||
Am J Physiol Heart Circ Physiol Homocysteine downregulates cardiac homeobox transcription factor NKX2.5 via IGFBP5. | ||
J Appl Toxicol The effect of maternal exposure to di-(2-ethylhexyl)-phthalate on fetal cardiac development in mice. | ||
Mol Med Rep H3K9me2 regulates early transcription factors to promote mesenchymal stem‑cell differentiation into cardiomyocytes. | ||
Int J Clin Exp Pathol SDF-1α promotes repair of myocardial ischemic necrosis zones in rats. |
