验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, THP-1 cells |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33052-1-AP targets NKX2-4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag37291 Product name: Recombinant human NKX2-4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 288-354 aa of NM_033176 Sequence: QNGASTPTPGQAGPQPPAPTPAPELEELSPSPPALHGPGGGLAALDAAAGEYSGGVLGANLLYGRTW 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NK2 homeobox 4 |
| 别名 | Homeobox protein NK-2 homolog D, Homeobox protein Nkx-2.4, NKX2D |
| 计算分子量 | 36kDa |
| 观测分子量 | 38-41 kDa |
| GenBank蛋白编号 | NM_033176 |
| 基因名称 | NKX2-4 |
| Gene ID (NCBI) | 644524 |
| RRID | AB_3742839 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9H2Z4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The NKX2-4 protein (full name: NK2 homeobox 4) belongs to the NK-2 type homeobox transcription factor family. Its main function is to act as a potential transcription factor, binding specifically to DNA sequences via its homeodomain and participating in the regulation of cell differentiation and the development of multicellular organisms. Studies have shown that this protein is active in the nucleus, and its aberrant expression may contribute to the development of acute myeloid leukemia by interfering with the normal differentiation of megakaryocytes and erythrocytes. Additionally, it can regulate the expression of genes such as TERT through allele-specific binding, thereby influencing tumor susceptibility in melanoma.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NKX2-4 antibody 33052-1-AP | Download protocol |
| WB protocol for NKX2-4 antibody 33052-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
