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验证数据展示

  • WB analysis of SH-SY5Y using 55484-1-AP

    WB analysis of SH-SY5Y using 55484-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55484-1-AP (NKX1-2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 55484-1-AP

    IHC staining of human brain using 55484-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55484-1-AP (NKX1-2 Antibody) at dilution of 1:50 (under 40x lens).

NKX1-2 Polyclonal antibody

NKX1-2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55484-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

bB238F13.2, C10orf121, Homeobox protein SAX 1, NK1 homeobox 2, NKX 1.1, NKX1 2, NKX1.1, NKX1-2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, mouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55484-1-AP targets NKX1-2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NK1 homeobox 2
别名 bB238F13.2, C10orf121, Homeobox protein SAX 1, NK1 homeobox 2, NKX 1.1, NKX1 2, NKX1.1, NKX1-2
计算分子量 32 kDa
观测分子量 32 kDa
GenBank蛋白编号NM_001146340
基因名称 NKX1-2
Gene ID (NCBI) 390010
RRIDAB_2881347
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UD57
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NKX1-2 antibody 55484-1-APDownload protocol
IHC protocol for NKX1-2 antibody 55484-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols