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验证数据展示

  • WB analysis using 28020-1-AP

    WB analysis using 28020-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28020-1-AP (NKPD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 28020-1-AP

    IHC staining of human liver cancer using 28020-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28020-1-AP (NKPD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 28020-1-AP

    IHC staining of human liver cancer using 28020-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28020-1-AP (NKPD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NKPD1 Polyclonal antibody

NKPD1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28020-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, ELISA

别名

FLJ33600, NKPD1, NKPD1 Ens

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, T-47D cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28020-1-AP targets NKPD1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag27667

Product name: Recombinant human NKPD1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 183-390 aa of XM_011526805

Sequence: MVSQRKKIERLVSREKFGSQLGFMCEVKKEVELLTDFLCFLEIYQRRRLRVVLEVTGLDTCYPERVVGVLNAINTLLSDSHAPFIFILVVDPSILAACLESAGNMKGTADNGYLFLNRTVTLPFSVPIMGRRTKLQFLHDAVQSRDDLLYREMTRKPWLPGDAGGESAQLLAVQAQAGTERGQGRIDDEAARRIQEALFCLHDERDCLY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NTPase, KAP family P-loop domain containing 1
别名 FLJ33600, NKPD1, NKPD1 Ens
计算分子量 68 aa
观测分子量70 kDa
GenBank蛋白编号XM_011526805
基因名称 NKPD1
Gene ID (NCBI) 284353
RRIDAB_2918141
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ17RQ9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for NKPD1 antibody 28020-1-APDownload protocol
WB protocol for NKPD1 antibody 28020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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