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NKPD1 Polyclonal antibody

NKPD1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 28020-1-AP

Print datasheet

Synonyms

FLJ33600, NKPD1, NKPD1 Ens

验证数据展示

  • WB analysis using 28020-1-AP

    WB analysis using 28020-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28020-1-AP (NKPD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 28020-1-AP

    IHC staining of human liver cancer using 28020-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28020-1-AP (NKPD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 28020-1-AP

    IHC staining of human liver cancer using 28020-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28020-1-AP (NKPD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMCF-7 cells, T-47D cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28020-1-AP targets NKPD1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human
Immunogen NKPD1 fusion protein Ag27667 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NTPase, KAP family P-loop domain containing 1
Synonyms FLJ33600, NKPD1, NKPD1 Ens
Calculated Molecular Weight 68 aa
Observed Molecular Weight70 kDa
GenBank Accession NumberXM_011526805
Gene Symbol NKPD1
Gene ID (NCBI) 284353
RRIDAB_2918141
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ17RQ9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NKPD1 antibody 28020-1-APDownload protocol
IHC protocol for NKPD1 antibody 28020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols