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验证数据展示

  • WB analysis using 11532-1-AP

    WB analysis using 11532-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11532-1-AP (NKG7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 11532-1-AP

    IHC staining of human colon cancer using 11532-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11532-1-AP (NKG7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NKG7 Polyclonal antibody

NKG7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11532-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

GMP 17, GIG-1 protein, GIG1, GIG 1 protein, G-CSF-induced gene 1 protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNK-92 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11532-1-AP targets NKG7 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 NKG7 fusion protein Ag2117 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 natural killer cell group 7 sequence
别名 GMP 17, GIG-1 protein, GIG1, GIG 1 protein, G-CSF-induced gene 1 protein
计算分子量 165 aa, 18 kDa
观测分子量18 kDa
GenBank蛋白编号BC015759
基因名称 NKG7
Gene ID (NCBI) 4818
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16617
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Natural killer cell granule protein 7 (NKG7) regulates lymphocyte granule exocytosis and downstream inflammation in a broad range of diseases. NKG7 expressed by CD4+ and CD8+ T cells were key in promoting inflammation during visceral leishmaniasis and malaria-two important parasitic diseases. It is expressed by natural killer cells and is critical for controlling cancer initiation, growth, and metastasis (PMID: 32839608; 8458737). In brief, NKG7 is a necessary T-cell intrinsic factor for the cytotoxic function of CD8+ T cells and established NKG7 as a new therapeutic target for cancer immunotherapy (PMID: 34911739).

实验方案

Product Specific Protocols
WB protocol for NKG7 antibody 11532-1-APDownload protocol
IHC protocol for NKG7 antibody 11532-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols