验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, NTERA-2 cl.D1 cells, rat testis tissue, PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26993-1-AP targets NKAPL in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag25779 Product name: Recombinant human NKAPL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 201-275 aa of BC101685 Sequence: YSDSDSNSESDTNSDSDDDKKRVKAKKKKKKKKHKTKKKKNKKTKKESSDSSCKDSEEDLSEATWMEQPNVADTM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NFKB activating protein-like |
| 别名 | C6orf194, NFKB activating protein like, NKAP like protein |
| 观测分子量 | 52 kDa, 65 kDa |
| GenBank蛋白编号 | BC101685 |
| 基因名称 | NKAPL |
| Gene ID (NCBI) | 222698 |
| RRID | AB_3742208 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5M9Q1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NKAPL (NF-kappa-B activating protein-like), also known as C6orf194, is a nuclear protein-coding gene located on chromosome 6p22.1. Initially identified as a germ cell-specific suppressor of Notch signaling essential for spermatogenesis, NKAPL has recently emerged as a tumor suppressor in non-small cell lung cancer through TRIM21 stabilization and NF-κB pathway inhibition. Additionally, NKAPL promoter methylation serves as a prognostic biomarker in multiple malignancies.(PMID: 25875095; 40605974)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NKAPL antibody 26993-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
