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验证数据展示

  • FC experiment of C57BL/6 mouse splenocytes using 65138-1-Ig

    FC experiment of C57BL/6 mouse splenocytes using 65138-1-Ig

    1X10^6 C57BL/6 mouse splenocytes were surface co-stained with APC Anti-Mouse CD49b (DX5) and 0.5 ug Anti-Mouse NK1.1 (CD161) (65138-1-Ig, Clone:PK136) and FITC anti-Mouse IgG2a Antibody at dilution 1:100, or 0.5 ug Mouse IgG2a Isotype Control (C1.18.4) and FITC anti-Mouse IgG2a Antibody at dilution 1:100. Cells were not fixed.

  • FC experiment of C57BL/6 mouse splenocytes using 65138-1-Ig

    FC experiment of C57BL/6 mouse splenocytes using 65138-1-Ig

    1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse NK1.1 (CD161) (65138-1-Ig, Clone:PK136) and FITC anti-Mouse IgG2a Antibody at dilution 1:100, or 0.5 ug Mouse IgG2a Isotype Control (C1.18.4) and FITC anti-Mouse IgG2a Antibody at dilution 1:100. Cells were not fixed.

Anti-Mouse NK1.1 (CD161) (PK136)

NK1.1 (CD161) Monoclonal Antibody for FC
Cat No. 65138-1-Ig

产品说明书

CloneNo. PK136

宿主/亚型

Mouse / IgG2a, kappa

种属反应性

Mouse

应用

FC

别名

CD161, Klrb1a, Ly55a, NK1.1, NK1.1 (CD161), NKR P1A, Nkrp1 a, NKRP12, Nkrp1a

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inC57BL/6 mouse splenocytes
Planning an IF experiment? We recommend our CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65138-1-Ig targets NK1.1 (CD161) in FC applications and shows reactivity with Mouse samples.

经测试应用 FC Application Description
经测试反应性 Mouse
免疫原 NK-1+ cells from mouse spleen and bone marrow 种属同源性预测
宿主/亚型 Mouse / IgG2a, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 killer cell lectin-like receptor subfamily B member 1A
别名 CD161, Klrb1a, Ly55a, NK1.1, NK1.1 (CD161), NKR P1A, Nkrp1 a, NKRP12, Nkrp1a
GenBank蛋白编号BC120707
基因名称 NK1.1
Gene ID (NCBI) 17057
RRIDAB_2918425
偶联类型 Unconjugated
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP27811
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

NK1.1 (CD161), also known as KLRB1 or NKR-P1A, is a type II transmembrane C-type lectin-like receptor and is expressed on the cell membrane as disulfide-linked homodimer (PMID: 8077657). It is expressed by the majority of NK cells and subsets of peripheral T cells, including both CD4+ and CD8+ T cells, and is expressed preferentially on adult T cells with a "memory" antigenic phenotype (PMID: 8077657; 22566826). Expression of CD161 correlates with the cytotoxic function of CD16+ NK cells, and ligation of CD161 with its ligand LLT1 inhibits NK cell cytotoxicity and cytokine secretion (PMID: 29686665).

实验方案

Product Specific Protocols
FC protocol for NK1.1 (CD161) antibody 65138-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols