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  • Featured Product
  • KD/KO Validated

NIT1 Polyclonal antibody

NIT1 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 14380-1-AP

Print datasheet

Synonyms

NIT1, nitrilase 1, Nitrilase homolog 1

验证数据展示

  • IHC staining of human pancreas cancer using 14380-1-AP

    IHC staining of human pancreas cancer using 14380-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14380-1-AP (NIT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 14380-1-AP

    IHC staining of human pancreas cancer using 14380-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14380-1-AP (NIT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 14380-1-AP

    IF Staining of HepG2 using 14380-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14380-1-AP (NIT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 14380-1-AP

    WB analysis using 14380-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14380-1-AP (NIT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, HEK-293T cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14380-1-AP targets NIT1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen NIT1 fusion protein Ag5764 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nitrilase 1
Synonyms NIT1, nitrilase 1, Nitrilase homolog 1
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 32-36 kDa
GenBank Accession NumberBC046149
Gene Symbol NIT1
Gene ID (NCBI) 4817
RRIDAB_2151314
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86X76
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NIT1 antibody 14380-1-APDownload protocol
IHC protocol for NIT1 antibody 14380-1-APDownload protocol
IF protocol for NIT1 antibody 14380-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cell Death Dis

NIT1 suppresses tumour proliferation by activating the TGFβ1-Smad2/3 signalling pathway in colorectal cancer.

Authors - Chun Lin
  • KD Validated
View Article