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NIPSNAP3A Polyclonal antibody

NIPSNAP3A Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 10751-1-AP

Print datasheet

Synonyms

HSPC299, NIPSNAP3A, NIPSNAP4, Protein NipSnap homolog 3A, Protein NipSnap homolog 4, TASSC

验证数据展示

  • WB analysis using 10751-1-AP

    WB analysis using 10751-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10751-1-AP (NIPSNAP3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 10751-1-AP

    IP experiment of mouse brain using 10751-1-AP

    IP result of anti-NIPSNAP3A (IP:10751-1-AP, 4ug; Detection:10751-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • IHC staining of human colon cancer using 10751-1-AP

    IHC staining of human colon cancer using 10751-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10751-1-AP (NIPSNAP3A Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon cancer using 10751-1-AP

    IHC staining of human colon cancer using 10751-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10751-1-AP (NIPSNAP3A Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HEK-293 using 10751-1-AP

    IF Staining of HEK-293 using 10751-1-AP

    Immunofluorescent analysis of HEK-293 cells using 10751-1-AP (NIPSNAP3A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, COLO 320 cells, HEK-293 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10751-1-AP targets NIPSNAP3A in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen NIPSNAP3A fusion protein Ag1143 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nipsnap homolog 3A (C. elegans)
Synonyms HSPC299, NIPSNAP3A, NIPSNAP4, Protein NipSnap homolog 3A, Protein NipSnap homolog 4, TASSC
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28-33 kDa
GenBank Accession NumberBC005935
Gene Symbol NIPSNAP3A
Gene ID (NCBI) 25934
RRIDAB_2298420
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UFN0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NIPSNAP3A antibody 10751-1-APDownload protocol
IHC protocol for NIPSNAP3A antibody 10751-1-APDownload protocol
IF protocol for NIPSNAP3A antibody 10751-1-APDownload protocol
IP protocol for NIPSNAP3A antibody 10751-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols