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NIP30 Polyclonal antibody

NIP30 Polyclonal Antibody for IF/ICC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 16830-1-AP

Print datasheet

Synonyms

C16orf94, CDA018, CDA10, FAM192A, NIP30, Protein FAM192A

验证数据展示

  • WB analysis of HeLa using 16830-1-AP

    WB analysis of HeLa using 16830-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16830-1-AP (NIP30 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 16830-1-AP

    IP experiment of HeLa using 16830-1-AP

    IP result of anti-NIP30 (IP:16830-1-AP, 4ug; Detection:16830-1-AP 1:500) with HeLa cells lysate 1080ug.

  • IF Staining of HeLa using 16830-1-AP

    IF Staining of HeLa using 16830-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16830-1-AP (NIP30 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

16830-1-AP targets NIP30 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen NIP30 fusion protein Ag10439 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NEFA-interacting nuclear protein NIP30
Synonyms C16orf94, CDA018, CDA10, FAM192A, NIP30, Protein FAM192A
Calculated Molecular Weight 254 aa, 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC071952
Gene Symbol NIP30
Gene ID (NCBI) 80011
RRIDAB_2109703
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9GZU8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NIP30, also known as NEFA-interacting nuclear protein 30, FAM192A (family with sequence similarity 192, member A), is a 254 amino acid nuclear protein encoded by a gene that maps to human chromosome 16q13.

实验方案

Product Specific Protocols
WB protocol for NIP30 antibody 16830-1-APDownload protocol
IF protocol for NIP30 antibody 16830-1-APDownload protocol
IP protocol for NIP30 antibody 16830-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

The REGγ inhibitor NIP30 increases sensitivity to chemotherapy in p53-deficient tumor cells.

Authors - Xiao Gao
View Article
humanWB

Retrovirology

Comparative host protein interactions with HTLV-1 p30 and HTLV-2 p28: insights into difference in pathobiology of human retroviruses.

Authors - Doueiri Rami R
View Article