• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Entactin Polyclonal antibody

Entactin Polyclonal Antibody for IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 13766-1-AP

Print datasheet

Synonyms

Entactin, NID, NID 1, NID1, Nidogen 1

验证数据展示

  • WB analysis using 13766-1-AP

    WB analysis using 13766-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13766-1-AP (Entactin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of human placenta using 13766-1-AP

    IP experiment of human placenta using 13766-1-AP

    IP result of anti-Entactin (IP:13766-1-AP, 4ug; Detection:13766-1-AP 1:200) with human placenta tissue lysate 4000ug.

  • IF Staining of HepG2 using 13766-1-AP

    IF Staining of HepG2 using 13766-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 13766-1-AP (Entactin antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman placenta tissue, mouse heart tissue, mouse placenta tissue
Positive IP detected inhuman placenta tissue
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13766-1-AP targets Entactin in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IP, WB, ELISA
Cited ApplicationsWB, IF, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Immunogen Entactin fusion protein Ag4749 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nidogen 1
Synonyms Entactin, NID, NID 1, NID1, Nidogen 1
Calculated Molecular Weight 1247 aa, 136 kDa
Observed Molecular Weight 136-150 kDa
GenBank Accession NumberBC045606
Gene Symbol NID1
Gene ID (NCBI) 4811
RRIDAB_10642564
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14543
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Entactin antibody 13766-1-APDownload protocol
IF protocol for Entactin antibody 13766-1-APDownload protocol
IP protocol for Entactin antibody 13766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB,CoIP

Bioact Mater

Nidogen1-enriched extracellular vesicles accelerate angiogenesis and bone regeneration by targeting Myosin-10 to regulate endothelial cell adhesion.

Authors - Pengzhen Cheng
View Article
humanIF

Matrix Biol

Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.

Authors - Emna Ouni
View Article