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验证数据展示

  • WB analysis using 86677-1-RR (same clone as 86677-1-PBS)

    WB analysis using 86677-1-RR (same clone as 86677-1-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 86677-1-RR (NHP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86677-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 86677-1-RR (same clone as 86677-1-PBS)

    IF Staining of HepG2 using 86677-1-RR (same clone as 86677-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NHP2 antibody (86677-1-RR, Clone: 251700B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86677-1-PBS in a different storage buffer formulation.

NHP2 Recombinant monoclonal antibody, PBS Only

NHP2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86677-1-PBS

产品说明书

CloneNo. 251700B11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

HSPC286, NHP 2, NHP2P, NOLA2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86677-1-PBS targets NHP2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag7251

Product name: Recombinant human NHP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-153 aa of BC000009

Sequence: MTKIKADPDGPEAQAEACSGERTYQELLVNQNPIAQPLASRRLTRKLYKCIKKAVKQKQIRRGVKEVQKFVNKGEKGIMVLAGDTLPIEVYCHLPVMCEDRNLPYVYIPSKTDLGAAAGSKRPTCVIMVKPHEEYQEAYDECLEEVQSLPLPL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NHP2 ribonucleoprotein homolog (yeast)
别名 HSPC286, NHP 2, NHP2P, NOLA2
计算分子量 17 kDa
观测分子量17-22 kDa
GenBank蛋白编号BC000009
基因名称 NHP2
Gene ID (NCBI) 55651
RRIDAB_3745048
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NX24
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NHP2 belongs to the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. It is required for ribosome biogenesis and telomere maintenance, and for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.

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