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NHLRC2 Polyclonal antibody

NHLRC2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29416-1-AP

Print datasheet

Synonyms

DKFZp779F115, FLJ20147, FLJ25621, FLJ33312, NHL repeat containing 2, NHLRC2

验证数据展示

  • WB analysis using 29416-1-AP

    WB analysis using 29416-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29416-1-AP (NHLRC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 29416-1-AP

    IHC staining of mouse brain using 29416-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29416-1-AP (NHLRC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 29416-1-AP

    IHC staining of mouse brain using 29416-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29416-1-AP (NHLRC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inU-251 cells, mouse spleen tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29416-1-AP targets NHLRC2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse
Immunogen NHLRC2 fusion protein Ag30159 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NHL repeat containing 2
Synonyms DKFZp779F115, FLJ20147, FLJ25621, FLJ33312, NHL repeat containing 2, NHLRC2
Calculated Molecular Weight 79 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberNM_198514
Gene Symbol NHLRC2
Gene ID (NCBI) 374354
RRIDAB_2918302
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NBF2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NHLRC2 antibody 29416-1-APDownload protocol
IHC protocol for NHLRC2 antibody 29416-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols