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验证数据展示

  • IHC staining of human brain using 24065-1-AP

    IHC staining of human brain using 24065-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24065-1-AP (NHEDC2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 24065-1-AP

    IHC staining of human brain using 24065-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24065-1-AP (NHEDC2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human plasma using 24065-1-AP

    WB analysis of human plasma using 24065-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 24065-1-AP (NHEDC2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

NHEDC2 Polyclonal antibody

NHEDC2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24065-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

NHA2, NHE10, NHEDC2, SLC9B2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24065-1-AP targets NHEDC2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 NHEDC2 fusion protein Ag21141 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Na+/H+ exchanger domain containing 2
别名 NHA2, NHE10, NHEDC2, SLC9B2
计算分子量 537 aa, 58 kDa
观测分子量 ~55 kDa
GenBank蛋白编号BC047447
基因名称 NHEDC2
Gene ID (NCBI) 133308
RRIDAB_2879423
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ86UD5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NHEDC2, also named as NHA2 and SLC9B2, contributes to the organellar volume homeostasis. It is required for osteoclast differentiation and bone resorption activity. NHEDC2 has two isoforms with MW 48 kDa and 58 kDa.

实验方案

Product Specific Protocols
WB protocol for NHEDC2 antibody 24065-1-APDownload protocol
IHC protocol for NHEDC2 antibody 24065-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols