验证数据展示
经过测试的应用
| Positive WB detected in | NCI-H1299 cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue |
Planning an IHC experiment? We recommend our IHCeasy NHE1/SLC9A1 Ready-To-Use IHC Kit. NHE1/SLC9A1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87385-1-RR targets NHE1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | solute carrier family 9 (sodium/hydrogen exchanger), member 1 |
| 别名 | SLC9A1, APNH, APNH1, FLJ42224, Na(+)/H(+) antiporter, amiloride-sensitive |
| 计算分子量 | 815 aa, 91 kDa |
| 观测分子量 | 90-100 kDa |
| GenBank蛋白编号 | BC012121 |
| 基因名称 | NHE1 |
| Gene ID (NCBI) | 6548 |
| RRID | AB_3745504 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P19634 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NHE1, also named as SLC9A1, encodes an Na+/H+ antiporter that is a membrane-bound enzyme involved in regulating pH of vertebrate cells. NHE1 regulates a number of cell behaviors, including adhesion, shape determination, migration, and proliferation (PMID:11807182). It is inhibited by the non-specific diuretic drug amiloride and activated by growth factors, mitogens, neurotransmitters, tumor promoters, and others (PMID: 2846238). NHE1 is expressed in heart where it contributes to cardiomyocyte pH homeostasis (PMID: 12832126, PMID: 23709596).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NHE1 antibody 87385-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
