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  • Featured Product
  • KD/KO Validated

NHE1 Monoclonal antibody

NHE1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3D9F3

Cat No : 67363-1-Ig

Print datasheet

Synonyms

APNH, APNH1, FLJ42224, Na(+)/H(+) exchanger 1, NHE 1, NHE1, SLC9A1, Sodium/hydrogen exchanger 1

验证数据展示

  • WB analysis using 67363-1-Ig

    WB analysis using 67363-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67363-1-Ig (NHE1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • IHC staining of human liver using 67363-1-Ig

    IHC staining of human liver using 67363-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67363-1-Ig (NHE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NHE1/SLC9A1 antibody included

经过测试的应用

Positive WB detected inA549 cells, NCI-H1299 cells, HEK-293 cells, LNCaP cells, Jurkat cells, HSC-T6 cells, PC-12 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NHE1/SLC9A1 Ready-To-Use IHC Kit. NHE1/SLC9A1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67363-1-Ig targets NHE1 in WB, IHC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, Rat
Cited Reactivityrat
Immunogen NHE1 fusion protein Ag14329 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name solute carrier family 9 (sodium/hydrogen exchanger), member 1
Synonyms APNH, APNH1, FLJ42224, Na(+)/H(+) exchanger 1, NHE 1, NHE1, SLC9A1, Sodium/hydrogen exchanger 1
Calculated Molecular Weight 815 aa, 91 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC012121
Gene Symbol SLC9A1
Gene ID (NCBI) 6548
RRIDAB_2882615
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP19634
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NHE1, also named as SLC9A1, is involved in pH regulation that can eliminate acids generated by active metabolism and counter adverse environmental conditions. NHE1 plays an important role in signal transduction. It has been shown that NHE1 participates in the development and progression of human breast cancer, gastric cancer and others. NHE1 has some isoforms with 91 kDa , 110 kDa (phosphoglycoprotein) and 210 kDa (dimeric protein). (PMID:28098891, 27751915, 8300588)

实验方案

Product Specific Protocols
WB protocol for NHE1 antibody 67363-1-IgDownload protocol
IHC protocol for NHE1 antibody 67363-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Acta Pharmacol Sin

Direct cardio-protection of Dapagliflozin against obesity-related cardiomyopathy via NHE1/MAPK signaling.

Authors - Ke Lin
  • KD Validated
View Article