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验证数据展示

  • WB analysis using 68593-1-Ig

    WB analysis using 68593-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68593-1-Ig (NGRN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

NGRN Monoclonal antibody

NGRN Monoclonal Antibody for WB, ELISA
Cat No. 68593-1-Ig

产品说明书

CloneNo. 2C2C9

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, ELISA

别名

DSC92, FI58G, Neugrin, NGRN

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68593-1-Ig targets NGRN in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 NGRN fusion protein Ag6790 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 neugrin, neurite outgrowth associated
别名 DSC92, FI58G, Neugrin, NGRN
计算分子量 32 kDa
观测分子量30-32 kDa
GenBank蛋白编号BC001682
基因名称 NGRN
Gene ID (NCBI) 51335
RRIDAB_3085292
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9NPE2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NGRN, also named as Neugrin or FI58G, is a 291 amino acid protein, which belongs to the neugrin family. NGRN localizes in the nucleus and is expressed at high levels in heart, brain and skeletal muscle. In brain, it is mainly expressed in neurons rather than glial cells. NGRN may be involved in neuronal differentiation.

实验方案

Product Specific Protocols
WB protocol for NGRN antibody 68593-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols