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验证数据展示

  • WB analysis using 32505-1-AP

    WB analysis using 32505-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32505-1-AP (NGFR/TNFRSF16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

NGFR/TNFRSF16 Polyclonal antibody

NGFR/TNFRSF16 Polyclonal Antibody for WB, ELISA
Cat No. 32505-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, ELISA

别名

CD271, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, Low-affinity nerve growth factor receptor p75NGFR, Low-affinity nerve growth factor receptor p75NGR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-12 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32505-1-AP targets NGFR/TNFRSF16 in WB, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nerve growth factor receptor (TNFR superfamily, member 16)
别名 CD271, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, Low-affinity nerve growth factor receptor p75NGFR, Low-affinity nerve growth factor receptor p75NGR
计算分子量46 kDa
观测分子量70 kDa
GenBank蛋白编号NM_033217.3
基因名称 Ngfr
Gene ID (NCBI) 18053
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9Z0W1-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NGFR, also named as TNFRSF16, Gp80-LNGFR, p75 ICD and CD271, is a low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. It mediates cell survival as well as cell death of neural cells. The antibody is specific to NGFR.

实验方案

Product Specific Protocols
WB protocol for NGFR/TNFRSF16 antibody 32505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols