验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, mouse brain tissue, rat brain tissue |
Planning an IHC experiment? We recommend our IHCeasy NGEF Ready-To-Use IHC Kit. NGEF primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30229-1-AP targets NGEF in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 |
CatNo: Ag30842 Product name: Recombinant human NGEF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 180-300 aa of BC031573 Sequence: QEYRDKSTLQEIETRRQQDAEIEDNTNGSPASEDTPEEEEEEEEEEEPASPPERKTLPQICLLSNPHSRFNLWQDLPEIRSSGVLEILQPEEIKLQEAMFELVTSEASYYKSLNLLVSHFM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | neuronal guanine nucleotide exchange factor |
| 别名 | EPHEXIN, Ephexin 1, Ephexin-1, NGEF |
| 计算分子量 | 710 aa, 82 kDa |
| 观测分子量 | 71 kDa |
| GenBank蛋白编号 | BC031573 |
| 基因名称 | NGEF |
| Gene ID (NCBI) | 25791 |
| RRID | AB_3086270 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N5V2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NGEF enables guanyl-nucleotide exchange factor activity. Predicted to be involved in several processes, including activation of GTPase activity; ephrin receptor signaling pathway; and negative regulation of dendritic spine morphogenesis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NGEF antibody 30229-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
