验证数据展示
经过测试的应用
Positive WB detected in | PC-3 cells, mouse brain tissue, rat brain tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30229-1-AP targets NGEF in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | Human, mouse, rat |
免疫原 | NGEF fusion protein Ag30842 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | neuronal guanine nucleotide exchange factor |
别名 | EPHEXIN, Ephexin 1, Ephexin-1, NGEF |
计算分子量 | 710 aa, 82 kDa |
观测分子量 | 71 kDa |
GenBank蛋白编号 | BC031573 |
基因名称 | NGEF |
Gene ID (NCBI) | 25791 |
RRID | AB_3086270 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q8N5V2 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NGEF enables guanyl-nucleotide exchange factor activity. Predicted to be involved in several processes, including activation of GTPase activity; ephrin receptor signaling pathway; and negative regulation of dendritic spine morphogenesis.
实验方案
Product Specific Protocols | |
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WB protocol for NGEF antibody 30229-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |