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NFYC Polyclonal antibody

NFYC Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 10129-2-AP

Print datasheet

Synonyms

CBF C, CBFC, H1TF2A, HAP5, HSM, NF YC, NFYC, Transactivator HSM 1/2

验证数据展示

  • WB analysis of K-562 using 10129-2-AP

    WB analysis of K-562 using 10129-2-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10129-2-AP (NFYC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10129-2-AP targets NFYC in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse
Immunogen NFYC fusion protein Ag0140 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear transcription factor Y, gamma
Synonyms CBF C, CBFC, H1TF2A, HAP5, HSM, NF YC, NFYC, Transactivator HSM 1/2
Calculated Molecular Weight 37 kDa, 50 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC005003
Gene Symbol NFYC
Gene ID (NCBI) 4802
RRIDAB_2152244
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13952
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NFYC, also named as Nuclear transcription factor Y subunit gamma or Transactivator HSM-1/2, is a 458 amino acid protein, which belongs to the NFYC/HAP5 subunit family. NFYC localizes in the nucleus and stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. SP1, NFYC and FOXO3, FOXO4 transcription factors are involved in the regulation of STK11 transcription.

实验方案

Product Specific Protocols
WB protocol for NFYC antibody 10129-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Res

Pancreas-specific Pten deficiency causes partial resistance to diabetes and elevated hepatic AKT signaling.

Authors - Tong Zan Z
View Article