验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, MCF-7 cells, RAW 264.7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15868-1-AP targets NFYB in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag8495 Product name: Recombinant human NFYB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-207 aa of BC005316 Sequence: MTMDGDSSTTDASQLGISADYIGGSHYVIQPHDDTEDSMNDHEDTNGSKESFREQDIYLPIANVARIMKNAIPQTGKIAKDAKECVQECVSEFISFITSEASERCHQEKRKTINGEDILFAMSTLGFDSYVEPLKLYLQKFREAMKGEKGIGGAVTATDGLSEELTEEAFTNQLPAGLITTDGQQQNVMVYTTSYQQISGVQQIQFS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nuclear transcription factor Y, beta |
| 别名 | CAAT box DNA-binding protein subunit B, CBF A, CBF B, CBFA, CBFB |
| 计算分子量 | 207 aa, 23 kDa |
| 观测分子量 | ~30 kDa |
| GenBank蛋白编号 | BC005316 |
| 基因名称 | NFYB |
| Gene ID (NCBI) | 4801 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P25208 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NFYB (Nuclear transcription factor Y subunit beta), also known as CBF-A/HAP3, is an essential subunit of the heterotrimeric NF-Y complex. NFYB has a histone-folding domain and binds NFYC in the cytoplasm; this dimer then enters the nucleus, recruits NFYA, and forms a trimer that specifically recognizes the CCAAT box, regulating genes involved in proliferation, apoptosis, and stress responses. (PMID: 37531316; PMID: 23977444)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NFYB antibody 15868-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
