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验证数据展示

  • WB analysis of A549 using 15370-1-AP

    WB analysis of A549 using 15370-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 15370-1-AP (NFS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 15370-1-AP

    WB analysis of HepG2 using 15370-1-AP

    WB result of NFS1 antibody (15370-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NFS1 transfected HepG2 cells.

  • IHC staining of human liver using 15370-1-AP

    IHC staining of human liver using 15370-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15370-1-AP (NFS1 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with NFS1 antibody included
  • IHC staining of human liver using 15370-1-AP

    IHC staining of human liver using 15370-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15370-1-AP (NFS1 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with NFS1 antibody included
  • IF Staining of HepG2 using 15370-1-AP

    IF Staining of HepG2 using 15370-1-AP

    Immunofluorescent analysis of HepG2 cells, using NFS1 antibody 15370-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of HepG2 using 15370-1-AP

    IP experiment of HepG2 using 15370-1-AP

    IP result of anti-NFS1 (IP:15370-1-AP, 4ug; Detection:15370-1-AP 1:500) with HepG2 cells lysate 3200ug.

  • IHC staining of human pancreas cancer using 15370-1-AP

    IHC staining of human pancreas cancer using 15370-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15370-1-AP (NFS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFS1 antibody included
  • IHC staining of human stomach cancer using 15370-1-AP

    IHC staining of human stomach cancer using 15370-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15370-1-AP (NFS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFS1 antibody included
  • IHC staining of mouse brain using 15370-1-AP

    IHC staining of mouse brain using 15370-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15370-1-AP (NFS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFS1 antibody included
  • IHC staining of mouse liver using 15370-1-AP

    IHC staining of mouse liver using 15370-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15370-1-AP (NFS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFS1 antibody included
  • IHC staining of rat brain using 15370-1-AP

    IHC staining of rat brain using 15370-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15370-1-AP (NFS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFS1 antibody included
  • IHC staining of rat liver using 15370-1-AP

    IHC staining of rat liver using 15370-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 15370-1-AP (NFS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFS1 antibody included
  • IHC staining of rat brain using 15370-1-AP

    IHC staining of rat brain using 15370-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15370-1-AP (NFS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFS1 antibody included
  • Featured Product
  • KD/KO Validated

NFS1 Polyclonal antibody

NFS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15370-1-AP

产品说明书

引用文献(18)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

别名

Cysteine desulfurase, EC:2.8.1.7, HUSSY 08, HUSSY-08, IscS

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, HeLa cells, human heart tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, human pancreas cancer tissue, human testis tissue, human ovary tissue, mouse liver tissue, rat liver tissue, human kidney tissue, human placenta tissue, rat brain tissue, human brain tissue, human stomach cancer tissue, human spleen tissue, mouse brain tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy® NFS1 Ready-To-Use IHC Kit. NFS1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15370-1-AP targets NFS1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, ChIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原

CatNo: Ag3872

Product name: Recombinant human NFS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-189 aa of BC018471

Sequence: MLLRAAWRRAAVAVTAAPGPKPAAPTRGLRLRVGDRAPQSAVPADTAAAPEVGPVLRPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGADPREIIFTSGATESNNIAIKTESHSVIQAGVQWHDLDSLQAPPPGFTPFSCLSLLSSWDYRRPPPHPANFLYF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NFS1 nitrogen fixation 1 homolog (S. cerevisiae)
别名 Cysteine desulfurase, EC:2.8.1.7, HUSSY 08, HUSSY-08, IscS
计算分子量 50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC018471
基因名称 NFS1
Gene ID (NCBI) 9054
RRIDAB_2151933
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y697
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NFS1(nitrogen fixation 1 homolog) is also named as NIFS, HUSSY-08 and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. The protein has been identified as a pyridoxal phosphate-containing homodimer that catalyzes the formation of equimolar amounts of elemental sulfur and L-alanine from the substrate, L-cysteine. It is reported that NFS1 is also able to catalyze the removal of selenium from selenocysteine, a mechanism similar to the L-cysteine reaction was postulated(PMID:9812986). It has 2 isoforms produced by alternative initiation.

实验方案

Product Specific Protocols
IF protocol for NFS1 antibody 15370-1-APDownload protocol
IHC protocol for NFS1 antibody 15370-1-APDownload protocol
IP protocol for NFS1 antibody 15370-1-APDownload protocol
WB protocol for NFS1 antibody 15370-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Res

NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly.

Authors - Tingting Hou
View Article
humanIHC

Redox Biol

Histone lactylation drives liver cancer metastasis by facilitating NSF1-mediated ferroptosis resistance after microwave ablation

Authors - Jiayan Huang
View Article
humanIHC

Redox Biol

NFS1, together with FXN, protects cells from ferroptosis and DNA damage in diffuse large B-cell lymphoma.

Authors - Xue Shi
View Article
mouseWB

J Nanobiotechnology

Cur@SF NPs alleviate Friedreich's ataxia in a mouse model through synergistic iron chelation and antioxidation.

Authors - Li Xu
View Article
mouseChIP

Adv Sci (Weinh)

NFS1 Regulates IDH2 to Attenuate Abdominal Aortic Aneurysms via Interacting With SP2.

Authors - Luzheng Zhang
  • KD Validated
View Article
mouseWB

Adv Sci (Weinh)

Impaired Iron-Sulfur Cluster Synthesis Induces Mitochondrial PARthanatos in Diabetic Cardiomyopathy

Authors - Mengyi Wang
View Article
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