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验证数据展示

  • WB analysis using 14086-1-AP

    WB analysis using 14086-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14086-1-AP (NFRKB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

NFRKB Polyclonal antibody

NFRKB Polyclonal Antibody for WB, ELISA
Cat No. 14086-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

DNA binding protein R kappa B, DNA-binding protein R kappa-B, INO80 complex subunit G, INO80G, Nuclear factor related to kappa-B-binding protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inBGC-823 cells, HepG2 cells, Jurkat cells, C2C12 cells, NIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14086-1-AP targets NFRKB in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5216

Product name: Recombinant human NFRKB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 949-1299 aa of BC063280

Sequence: GKDVLRLPPSSITTDAKGQTVLRITPDMMATLAKSQVTTVKLTQDLFGTGGNTTGKGISATLHVTSNPVHAADSPAKASSASAPSSTPTGTTVVKVTPDLKPTEASSSAFRLMPALGVSVADQKGKSTVASSEAKPAATIRIVQGLGVMPPKAGQTITVATHAKQGASVASGSGTVHTSAVSLPSMNAAVSKTVAVASGAASTPISISTGAPTVRQVPVSTTVVSTSQAEKLPTRITVPLSVISQPMKGKSVVTAPIIKGNLGADLSGLGRNIILTTMPAGTKLIAGNKPVSFLTAQQLQQLQQQGQATQVRIQTVPASHLQQGTASGSSKAVSTVVVTTAPSPKQAPEQQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear factor related to kappaB binding protein
别名 DNA binding protein R kappa B, DNA-binding protein R kappa-B, INO80 complex subunit G, INO80G, Nuclear factor related to kappa-B-binding protein
计算分子量 1299 aa, 139 kDa
观测分子量 160 kDa
GenBank蛋白编号BC063280
基因名称 NFRKB
Gene ID (NCBI) 4798
RRIDAB_3669177
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6P4R8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NFRKB (Nuclear Factor Related to KappaB Binding Protein) plays a role in the recombination of DNA, it helps in the regulation of transcription, DNA-templated, and transcription by RNA polymerase II, and is involved in the regulation of inflammatory responses. It also regulates protein deubiquitination, which is important for protein stability and function.

实验方案

Product Specific Protocols
WB protocol for NFRKB antibody 14086-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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