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验证数据展示

  • WB analysis using 15672-1-AP

    WB analysis using 15672-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15672-1-AP (NFKBID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

NFKBID Polyclonal antibody

NFKBID Polyclonal Antibody for WB, ELISA
Cat No. 15672-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

I kappa B delta, I-kappa-B-delta, IkappaBNS, IkB delta, IkB-delta

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA20 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15672-1-AP targets NFKBID in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag8139

Product name: Recombinant human NFKBID protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-150 aa of BC006273

Sequence: MRSCCVVQAGLQLLASSDPPSSASQSARITGVNHRTPPREIKSNKTVLHLAVQAANPTLVQLLLELPRGDLRTFVNMKAHGNTALHMAAALPPGPAQEAIVRHLLAAGADPTLRNLENEQPVHLLRPGPGPEGLRQLLKRSRVAPPGLSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, delta
别名 I kappa B delta, I-kappa-B-delta, IkappaBNS, IkB delta, IkB-delta
计算分子量 150 aa, 16 kDa
观测分子量 33 kDa
GenBank蛋白编号BC006273
基因名称 NFKBID
Gene ID (NCBI) 84807
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NI38
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NFKBID, also known as IkBNS, belongs to the IKB protein family of NFKB inhibitors and is induced by T-cell receptor stimulation(PMID: 11931770). NFKBID regulates the expression of IL-2, IL-6, and other cytokines through regulation on NF-kappa-B activity. NFKBID plays a role in regulating inflammatory responses. NFKBID is involved in the induction of T helper 17 cells (Th17) differentiation upon antigen recognition by T cell antigen receptor (TCR). (PMID: 25347393)

实验方案

Product Specific Protocols
WB protocol for NFKBID antibody 15672-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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