验证数据展示

WB analysis using 10268-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
FC experiment of HeLa using 10268-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human IkB Alpha (10268-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IHC staining of human stomach using 10268-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 10x lens).
Ready-to-use IHC kits available with NFKBIA antibody included
IHC staining of human stomach using 10268-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 40x lens).
Ready-to-use IHC kits available with NFKBIA antibody included
IF Staining of HeLa using 10268-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IkB Alpha antibody (10268-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IHC staining of mouse kidney using 10268-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with NFKBIA antibody included
IHC staining of mouse kidney using 10268-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with NFKBIA antibody included
IP experiment of HeLa using 10268-1-AP
IP result of anti-IkB Alpha (IP:10268-1-AP, 4ug; Detection:10268-1-AP 1:15000) with HeLa cells lysate 1320 ug.
IHC staining of rat stomach using 10268-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with NFKBIA antibody included
IHC staining of rat stomach using 10268-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with NFKBIA antibody included
IF Staining of A431 using 10268-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using IkB Alpha antibody (10268-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of HeLa using 10268-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IkB Alpha antibody (10268-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
WB analysis using 10268-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB Alpha antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

IkB Alpha Polyclonal antibody

IkB Alpha Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10268-1-AP

产品说明书

引用文献(591)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig and More (3)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

别名

IKBA, IκB α, NFKBIA, I kappa B alpha, IkappaBalpha

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (591)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	A431 cells, HeLa cells, HEK-293 cells, pig liver tissue, HL-60 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, PC-12 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human stomach tissue, human kidney tissue, human prostate cancer tissue, mouse kidney tissue, rat stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, A431 cells
Positive FC (Intra) detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy NFKBIA Ready-To-Use IHC Kit. NFKBIA primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 1 publications below
WB	See 568 publications below
IHC	See 15 publications below
IF	See 8 publications below
CoIP	See 2 publications below

产品信息

10268-1-AP targets IkB Alpha in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF, CoIP
经测试反应性	human, mouse, rat, pig
文献引用反应性	human, mouse, rat, pig, chicken, bovine, ducks
免疫原	IkB Alpha fusion protein Ag0345 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
别名	IKBA, IκB α, NFKBIA, I kappa B alpha, IkappaBalpha
计算分子量	36 kDa
观测分子量	36 kDa
GenBank蛋白编号	BC002601
基因名称	IkB Alpha
Gene ID (NCBI)	4792
RRID	AB_2151423
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P25963
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

实验方案

Product Specific Protocols
WB protocol for IkB Alpha antibody 10268-1-AP	Download protocol
IHC protocol for IkB Alpha antibody 10268-1-AP	Download protocol
IF protocol for IkB Alpha antibody 10268-1-AP	Download protocol
IP protocol for IkB Alpha antibody 10268-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Cell Metab Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer Authors - Yu Jiang View Article
human	IF	Nat Cell Biol Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function. Authors - Li Wang View Article
human	WB	Cancer Commun (Lond) Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer Authors - Xingbin Hu View Article
mouse	WB	Mol Cell Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation Authors - Chuanlong Wang View Article
mouse	WB	Adv Sci (Weinh) Reprogramming Lung Redox Homeostasis by NIR Driven Ultra-Small Pd Loaded Covalent Organic Framework Inhibits NF-κB Pathway for Acute Lung Injury Immunotherapy Authors - Doudou Lei View Article
human	WB,IF	Nucleic Acids Res p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway Authors - Xinxing Zhu View Article

验证数据展示

WB analysis using 10268-1-AP

FC experiment of HeLa using 10268-1-AP

IHC staining of human stomach using 10268-1-AP

IHC staining of human stomach using 10268-1-AP

IF Staining of HeLa using 10268-1-AP

IHC staining of mouse kidney using 10268-1-AP

IHC staining of mouse kidney using 10268-1-AP

IP experiment of HeLa using 10268-1-AP

IHC staining of rat stomach using 10268-1-AP

IHC staining of rat stomach using 10268-1-AP

IF Staining of A431 using 10268-1-AP

IF Staining of HeLa using 10268-1-AP

WB analysis using 10268-1-AP

IkB Alpha Polyclonal antibody

IkB Alpha Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer

Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.

Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer

Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation

Reprogramming Lung Redox Homeostasis by NIR Driven Ultra-Small Pd Loaded Covalent Organic Framework Inhibits NF-κB Pathway for Acute Lung Injury Immunotherapy

p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway