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验证数据展示

  • WB analysis using 84022-5-RR (same clone as 84022-5-PBS)

    WB analysis using 84022-5-RR (same clone as 84022-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84022-5-RR (NFKB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84022-5-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 84022-5-RR (same clone as 84022-5-PBS)

    FC experiment of A431 using 84022-5-RR (same clone as 84022-5-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug NFKB2,p100 Recombinant antibody (84022-5-RR, Clone:241126C9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84022-5-PBS in a different storage buffer formulation.

  • WB analysis of HAP1 using 84022-5-RR (same clone as 84022-5-PBS)

    WB analysis of HAP1 using 84022-5-RR (same clone as 84022-5-PBS)

    WB result of NFKB2,p100 antibody (84022-5-RR; 1:60000; room temperature for 1.5 hours) with wild-type and sg-NFKB2 transfected HAP1 cells. This data was developed using the same antibody clone with 84022-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84022-5-RR

    Affinity and Kinetic Characterization of 84022-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84022-5-RR against Human NFKB2,p100 were performed. The affinity constant is 0.109 nM.

  • Featured Product
  • KD/KO验证
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NFKB2,p100 Recombinant monoclonal antibody, PBS Only

NFKB2,p100 Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84022-5-PBS

产品说明书

CloneNo. 241126C9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

NFKB2, 241126C9, DNA binding factor KBF2, DNA-binding factor KBF2, H2TF1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NFKB2 Ready-To-Use IHC Kit. NFKB2 primary antibody included.

产品信息

84022-5-PBS targets NFKB2,p100 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0662

Product name: Recombinant human NFKB2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 600-899 aa of BC002844

Sequence: GLYPVHLAVRARSPECLDLLVDSGAEVEATERQGGRTALHLATEMEELGLVTHLVTKLRANVNARTFAGNTPLHLAAGLGYPTLTRLLLKAGADIHAENEEPLCPLPSPPTSDSDSDSEGPEKDTRSSFRGHTPLDLTCSTKVKTLLLNAAQNTMEPPLTPPSPAGPGLSLGDTALQNLEQLLDGPEAQGSWAELAERLGLRSLVDTYRQTTSPSGSLLRSYELAGGDLAGLLEALSDMGLEEGVRLLRGPETRDKLPSTEVKEDSAYGSQSVEQEAEKLGPPPEPPGGLCHGHPQPQVH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
别名 NFKB2, 241126C9, DNA binding factor KBF2, DNA-binding factor KBF2, H2TF1
计算分子量 97 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC002844
基因名称 NFKB2
Gene ID (NCBI) 4791
ENSEMBL Gene IDENSG00000077150
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ00653
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. This antibody can bind p100 isoform of NFKB2 spefically, but not p52 isoform.