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验证数据展示

  • WB analysis using 67983-1-Ig (same clone as 67983-1-PBS)

    WB analysis using 67983-1-Ig (same clone as 67983-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67983-1-Ig (NFIX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67983-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51754-1

    Cytometric bead array sample test of MP51754-1

    Cytometric bead array sample test of MP51754-1, NFIX Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67983-2-PBS. Detection antibody: 67983-1-PBS.

  • WB analysis of HeLa using 67983-1-Ig (same clone as 67983-1-PBS)

    WB analysis of HeLa using 67983-1-Ig (same clone as 67983-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67983-1-Ig (NFIX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67983-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67983-1-Ig (same clone as 67983-1-PBS)

    IHC staining of mouse brain using 67983-1-Ig (same clone as 67983-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67983-1-Ig (NFIX antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67983-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFIX antibody included
  • IHC staining of mouse brain using 67983-1-Ig (same clone as 67983-1-PBS)

    IHC staining of mouse brain using 67983-1-Ig (same clone as 67983-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67983-1-Ig (NFIX antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67983-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFIX antibody included
  • IHC staining of rat brain using 67983-1-Ig (same clone as 67983-1-PBS)

    IHC staining of rat brain using 67983-1-Ig (same clone as 67983-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67983-1-Ig (NFIX antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67983-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFIX antibody included
  • IHC staining of rat brain using 67983-1-Ig (same clone as 67983-1-PBS)

    IHC staining of rat brain using 67983-1-Ig (same clone as 67983-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67983-1-Ig (NFIX antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67983-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFIX antibody included
  • Cytometric bead array standard curve of MP51754-1

    Cytometric bead array standard curve of MP51754-1

    Cytometric bead array standard curve of MP51754-1, NFIX Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67983-2-PBS. Detection antibody: 67983-1-PBS. Standard:Ag31447. Range: 0.313-40 ng/mL.

NFIX Monoclonal antibody, PBS Only (Detector)

NFIX Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 67983-1-PBS

产品说明书

CloneNo. 1E8A1

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

1E8A1, CCAAT-box-binding transcription factor, CTF, NF1-X, NF-I/X

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NFIX Ready-To-Use IHC Kit. NFIX primary antibody included.

产品信息

67983-1-PBS targets NFIX as part of a matched antibody pair:

MP51754-1: 67983-2-PBS capture and 67983-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag31447

Product name: Recombinant human NFIX protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 246-400 aa of NM_001271043

Sequence: ADLESPSYYNINQVTLGRRSITSPPSTSTTKRPKSIDDSEMESPVDDVFYPGTGRSPAAGSSQSSGWPNDVDAGPASLKKSGKLDFCSALSSQGSSPRMAFTHHPLPVLAGVRPGSPRATASALHFPSTSIIQQSSPYFTHPTIRYHHHHGQDSL

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 nuclear factor I/X (CCAAT-binding transcription factor)
别名 1E8A1, CCAAT-box-binding transcription factor, CTF, NF1-X, NF-I/X
计算分子量 50 kDa
观测分子量45kDa, 50kDa
GenBank蛋白编号NM_001271043
基因名称 NFIX
Gene ID (NCBI) 4784
RRIDAB_2918732
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ14938
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NFIX, also named CTF, belongs to the CTF/NF-I family. NFIX recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. NFIX is individually capable of activating transcription and replication.

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