验证数据展示
产品信息
CL488-66999 targets NFIA in applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| 经测试应用 | Application Description |
| 经测试反应性 | human, mouse, rat, pig, rabbit |
| 免疫原 | NFIA fusion protein Ag17873 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | nuclear factor I/A |
| 别名 | NF1 A, NF I/A, KIAA1439, CTF, CCAAT-box-binding transcription factor |
| 计算分子量 | 498 aa, 55 kDa |
| 观测分子量 | 60-70 kDa |
| GenBank蛋白编号 | BC022264 |
| 基因名称 | NFIA |
| Gene ID (NCBI) | 4774 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q12857 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The NFI (nuclear factor I) family consists of four members in vertebrates (NFI-A, NFI-B, NFI-C and NFI-X), and the four NFI genes are expressed in unique patterns during mouse embryogenesis and in the adult. Four isoforms of NFIA were found in human and they play various roles in DNA replication, DNA-dependent transcritpion via their DNA binding property. Multiple residues of NFIA can be phosphorylated resulting in mild shifts of its practical molecular weight. Recent finding also revealed its neuroprotective function in NMDA-induced neuronal damage. The calculated molecular weight of NFIA is 55 kDa, However the size of the proteins cross-linked to the adenoviral NF-I element ranged from 60 to 80 kDa . The larger size observed by us could be due to the oligo protein complex, which would increase the size by 15-20 kDa. (PMID: 11447215)