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NFE2L1 Polyclonal antibody

NFE2L1 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IF/ICC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 17062-1-AP

Print datasheet

Synonyms

HBZ17, LCR F1, Locus control region factor 1, NF E2 related factor 1, NFE2 related factor 1, NFE2L1, NRF1, TCF 11, TCF11, Transcription factor 11, Transcription factor HBZ17, Transcription factor LCR F1

验证数据展示

  • WB analysis of HEK-293 using 17062-1-AP

    WB analysis of HEK-293 using 17062-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17062-1-AP (NFE2L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 17062-1-AP

    IF Staining of HepG2 using 17062-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NFE2L1 antibody (17062-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHEK-293 cells, NIH/3T3 cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17062-1-AP targets NFE2L1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Immunogen NFE2L1 fusion protein Ag10832 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear factor (erythroid-derived 2)-like 1
Synonyms HBZ17, LCR F1, Locus control region factor 1, NF E2 related factor 1, NFE2 related factor 1, NFE2L1, NRF1, TCF 11, TCF11, Transcription factor 11, Transcription factor HBZ17, Transcription factor LCR F1
Calculated Molecular Weight 772 aa, 85 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC010623
Gene Symbol NFE2L1
Gene ID (NCBI) 4779
RRIDAB_2878342
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14494
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NFE2L1, also named as HBZ17, NRF1, TCF11 and LCR-F1, belongs to the bZIP family and CNC subfamily. It activates erythroid-specific, globin gene expression. NFE2L1 is involved in mediating ascorbic acid-induced osterix expression and osteoblast differentiation via binding to the antioxidant response element of the osterix promoter. NFE2L1, produced by osteoblasts, is involved in regulating osterix expression, osteoblast differentiation, and bone formation. (PMID:19887580). This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human ESRRG.

实验方案

Product Specific Protocols
WB protocol for NFE2L1 antibody 17062-1-APDownload protocol
IF protocol for NFE2L1 antibody 17062-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Overexpression of NRF1-742 or NRF1-772 Reduces Arsenic-Induced Cytotoxicity and Apoptosis in Human HaCaT Keratinocytes.

Authors - Shuai Wang
View Article
ratWB,IHC

Stem Cell Rev Rep

EIF4A3-Induced Exosomal circLRRC8A Alleviates Granulosa Cells Senescence Via the miR-125a-3p/NFE2L1 axis

Authors - Jie Xing
View Article