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验证数据展示

  • WB analysis using 12936-1-AP (same clone as 12936-1-PBS)

    WB analysis using 12936-1-AP (same clone as 12936-1-PBS)

    Untreated and MG132 treated HEK-293T were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12936-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 12936-1-AP (same clone as 12936-1-PBS)

    IHC staining of human breast cancer using 12936-1-AP (same clone as 12936-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12936-1-AP (NFE2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12936-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse skeletal muscle using 12936-1-AP (same clone as 12936-1-PBS)

    IP experiment of mouse skeletal muscle using 12936-1-AP (same clone as 12936-1-PBS)

    IP result of anti-NFE2L1 (IP:12936-1-AP, 4ug; Detection:12936-1-AP 1:500) with mouse skeletal muscle tissue lysate 2200ug. This data was developed using the same antibody clone with 12936-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NFE2L1 Polyclonal antibody, PBS Only

NFE2L1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12936-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

Endoplasmic reticulum membrane sensor NFE2L1, HBZ17, LCR-F1, Locus control region-factor 1, NF-E2-related factor 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12936-1-PBS targets NFE2L1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3916

Product name: Recombinant human NFE2L1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 293-742 aa of BC010623

Sequence: MQAMEVNTSASEILYSAPPGDPLSTNYSLAPNTPINQNVSLHQASLGGCSQDFLLFSPEVESLPVASSSTLLPLAPSNSTSLNSTFGSTNLTGLFFPPQLNGTANDTAGPELPDPLGGLLDEAMLDEISLMDLAIEEGFNPVQASQLEEEFDSDSGLSLDSSHSPSSLSSSEGSSSSSSSSSSSSSSASSSASSSFSEEGAVGYSSDSETLDLEEAEGAVGYQPEYSKFCRMSYQDPAQLSCLPYLEHVGHNHTYNMAPSALDSADLPPPSALKKGSKEKQADFLDKQMSRDEHRARAMKIPFTNDKIINLPVEEFNELLSKYQLSEAQLSLIRDIRRRGKNKMAAQNCRKRKLDTILNLERDVEDLQRDKARLLREKVEFLRSLRQMKQKVQSLYQEVFGRLRDENGRPYSPSQYALQYAGDGSVLLIPRTMADQQARRQERKPKDRRK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear factor (erythroid-derived 2)-like 1
别名 Endoplasmic reticulum membrane sensor NFE2L1, HBZ17, LCR-F1, Locus control region-factor 1, NF-E2-related factor 1
计算分子量 772 aa, 85 kDa
观测分子量 65-120 kDa
GenBank蛋白编号BC010623
基因名称 NFE2L1
Gene ID (NCBI) 4779
RRIDAB_2267298
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14494
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NFE2L1, also named as HBZ17, NRF1, TCF11 and LCR-F1, belongs to the bZIP family and CNC subfamily. It activates erythroid-specific, globin gene expression. NFE2L1 is involved in mediating ascorbic acid-induced osterix expression and osteoblast differentiation via binding to the antioxidant response element of the osterix promoter. NFE2L1, produced by osteoblasts, is involved in regulating osterix expression, osteoblast differentiation, and bone formation. (PMID:19887580).

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