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NFE2 Monoclonal antibody

NFE2 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

1D7C8

Cat No : 66436-1-Ig

Print datasheet

Synonyms

Leucine zipper protein NF E2, NF E2, NFE2, p45, p45 NF E2

验证数据展示

  • WB analysis of HL-60 using 66436-1-Ig

    WB analysis of HL-60 using 66436-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 66436-1-Ig (NFE2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHL-60 cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy NFE2 Ready-To-Use IHC Kit. NFE2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66436-1-Ig targets NFE2 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB,ELISA
Tested Reactivity human
Immunogen NFE2 fusion protein Ag1570 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nuclear factor (erythroid-derived 2), 45kDa
Synonyms Leucine zipper protein NF E2, NF E2, NFE2, p45, p45 NF E2
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC005044
Gene Symbol NFE2
Gene ID (NCBI) 4778
RRIDAB_2881806
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16621
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The transcription factor p45 nuclear factor-erythroid-derived 2(NFE2) is one component of the NF-E2 complex that essential for regulating reythroid and megakaryocytic maturation and differentiation. It also plays crucial roles in erythroid and megakaryocytic lineages. NFE2 recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters, and acts as a activator. And it may has a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.

实验方案

Product Specific Protocols
WB protocol for NFE2 antibody 66436-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols