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验证数据展示

  • WB analysis using 11667-1-AP

    WB analysis using 11667-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11667-1-AP (NFATC2IP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 11667-1-AP

    IF Staining of HeLa using 11667-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NFATC2IP antibody (11667-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

NFATC2IP Polyclonal antibody

NFATC2IP Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 11667-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Nuclear factor of activated T-cells, cytoplasmic 2-interacting protein, NIP45, NFATC2-interacting protein, NFATC2 interacting protein, 45 kDa NFAT-interacting protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, Raji cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11667-1-AP targets NFATC2IP in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2217

Product name: Recombinant human NFATC2IP protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-218 aa of BC021551

Sequence: PPSPRTKSRTHTRALKKLSEVNKRLQDLRSCLSPKPPQGQEQQGQEDEVVLVEGPTLPETPRLFPLKIRCRADLVRLPLRMSEPLQSVVDHMATHLGVSPSRILLLFGETELSPTATPRTLKLGVADIIDCVVLTSSPEATETSQQLQLRVQGKEKHQTLEVSLSRDSPLKTLMSHYEEAMGLSGRKLSFFFDGTKLSGRELPADLGMESGDLIEVWG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 interacting protein
别名 Nuclear factor of activated T-cells, cytoplasmic 2-interacting protein, NIP45, NFATC2-interacting protein, NFATC2 interacting protein, 45 kDa NFAT-interacting protein
计算分子量 138 aa, 15 kDa
观测分子量 50-65 kDa
GenBank蛋白编号BC021551
基因名称 NFATC2IP
Gene ID (NCBI) 84901
RRIDAB_3669129
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NCF5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NFATC2IP, also named as NIP45, is a 419 amino acid protein, which contains one ubiquitin-like domain. TRAF1 is associated with a fraction of NFATC2IP in the cytoplasm and prevents its translocation to the nucleus. NFATC2IP In T-helper 2 (Th2) cells, regulates the magnitude of NFAT-driven transcription of a specific subset of cytokine genes, including IL3, IL4, IL5 and IL13, but not IL2.

实验方案

Product Specific Protocols
IF protocol for NFATC2IP antibody 11667-1-APDownload protocol
WB protocol for NFATC2IP antibody 11667-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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