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验证数据展示

  • WB analysis using 66963-1-Ig (same clone as 66963-1-PBS)

    WB analysis using 66963-1-Ig (same clone as 66963-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66963-1-Ig (NFATC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66963-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 66963-1-Ig (same clone as 66963-1-PBS)

    IHC staining of human urothelial carcinoma using 66963-1-Ig (same clone as 66963-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66963-1-Ig (NFATC1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66963-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 66963-1-Ig (same clone as 66963-1-PBS)

    IHC staining of human urothelial carcinoma using 66963-1-Ig (same clone as 66963-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66963-1-Ig (NFATC1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66963-1-PBS in a different storage buffer formulation.

  • WB analysis using 66963-1-Ig (same clone as 66963-1-PBS)

    WB analysis using 66963-1-Ig (same clone as 66963-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66963-1-Ig (NFATC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66963-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

NFATC1 Monoclonal antibody, PBS Only

NFATC1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66963-1-PBS

产品说明书

CloneNo. 1E1B10

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

1E1B10, NFAT2, NFATc, NF-ATc, NF-ATc1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66963-1-PBS targets NFATC1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag13014

Product name: Recombinant human NFATC1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-351 aa of BC104753

Sequence: MPSTSFPVPSKFPLGPAAAVFGRGETLGPAPRAGGTMKSAEEEHYGYASSNVSPALPLPTAHSTLPAPCHNLQTSTPGIIPPADHPSGYGAALDGGPAGYFLSSGHTRPDGAPALESPRIEITSCLGLYHNNNQFFHDVEVEDVLPSSKRSPSTATLSLPSLEAYRDPSCLSPASSLSSRSCNSEASSYESNYSYPYASPQTSPWQSPCVSPKTTDPEEGFPRGLGACTLLGSPRHSPSTSPRASVTEESWLGARSSRPASPCNKRKYSLNGRQPPYSPHHSPTPSPHGSPRVSVTDDSWLGNTTQYTSSAIVAAINALTTDSSLDLGDGVPVKSRKTTLEQPPSVALKVE

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
别名 1E1B10, NFAT2, NFATc, NF-ATc, NF-ATc1
计算分子量716aa,78 kDa; 943aa,101 kDa
观测分子量 101 kDa
GenBank蛋白编号BC104753
基因名称 NFATC1
Gene ID (NCBI) 4772
RRIDAB_2882286
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO95644
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NFATc1(nuclear factor-activated T cells c1) is one of the transcription factors family consisting 5 members which were considered as a regulator of T-cell activation. In immune cell, NFATc1 is located in the cytoplasm in a hyperphosphorylated state and translocates into the nucleus upon cell stimulation via dephosphorylation(PMID:28327458). One paper observed that ectopic expression and nuclear accumulation of NFATc1 in more than 60% of human pancreatic cancers (PMID:25623042). Several alternative splicing transcript variants encoding different isoforms have been identified for this gene. And different isoforms of this protein may regulate different cytokine gene expression. NFATc1 is a main transcriptional activator for cytokine genes in activated lymphoid cells. Also some studies demonstrated that NFATc1 plays the role of a master transcription regulator of osteoclast differentiation (PMID:25489571).