• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66963-1-Ig (same clone as 66963-1-PBS)

    WB analysis using 66963-1-Ig (same clone as 66963-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66963-1-Ig (NFATC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66963-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 66963-1-Ig (same clone as 66963-1-PBS)

    IHC staining of human urothelial carcinoma using 66963-1-Ig (same clone as 66963-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66963-1-Ig (NFATC1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66963-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 66963-1-Ig (same clone as 66963-1-PBS)

    IHC staining of human urothelial carcinoma using 66963-1-Ig (same clone as 66963-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66963-1-Ig (NFATC1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66963-1-PBS in a different storage buffer formulation.

  • WB analysis using 66963-1-Ig (same clone as 66963-1-PBS)

    WB analysis using 66963-1-Ig (same clone as 66963-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66963-1-Ig (NFATC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66963-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

NFATC1 Monoclonal antibody, PBS Only

NFATC1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66963-1-PBS

产品说明书

CloneNo. 1E1B10

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

Nuclear factor of activated T cells, cytoplasmic 1, NF-ATc1, NF-ATc, NFATc, NFAT2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

66963-1-PBS targets NFATC1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 NFATC1 fusion protein Ag13014 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
别名 Nuclear factor of activated T cells, cytoplasmic 1, NF-ATc1, NF-ATc, NFATc, NFAT2
计算分子量716aa,78 kDa; 943aa,101 kDa
观测分子量 101 kDa
GenBank蛋白编号BC104753
基因名称 NFATC1
Gene ID (NCBI) 4772
RRIDAB_2882286
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95644
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NFATc1(nuclear factor-activated T cells c1) is one of the transcription factors family consisting 5 members which were considered as a regulator of T-cell activation. In immune cell, NFATc1 is located in the cytoplasm in a hyperphosphorylated state and translocates into the nucleus upon cell stimulation via dephosphorylation(PMID:28327458). One paper observed that ectopic expression and nuclear accumulation of NFATc1 in more than 60% of human pancreatic cancers (PMID:25623042). Several alternative splicing transcript variants encoding different isoforms have been identified for this gene. And different isoforms of this protein may regulate different cytokine gene expression. NFATc1 is a main transcriptional activator for cytokine genes in activated lymphoid cells. Also some studies demonstrated that NFATc1 plays the role of a master transcription regulator of osteoclast differentiation (PMID:25489571).