验证数据展示
经过测试的应用
| Positive WB detected in | rat brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
26351-1-AP targets Neurofascin in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, rat, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag24165 Product name: Recombinant human NFASC protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 74-185 aa of BC137013 Sequence: RFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | neurofascin homolog (chicken) |
| 别名 | KIAA0756, Neurofascin, neurofascin homolog (chicken), NFASC, NRCAML |
| 计算分子量 | 1347 aa, 150 kDa |
| 观测分子量 | 186 kDa |
| GenBank蛋白编号 | BC137013 |
| 基因名称 | Neurofascin |
| Gene ID (NCBI) | 23114 |
| RRID | AB_2880486 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O94856 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Neurofascin antibody 26351-1-AP | Download protocol |
| WB protocol for Neurofascin antibody 26351-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) Tumor-suppressive miR-3650 inhibits tumor metastasis by directly targeting NFASC in hepatocellular carcinoma. | ||
J Cancer Prev Gene Expression Changes by Diallyl Trisulfide Administration in Chemically-induced Mammary Tumors in Rats. | ||
Cell Rep Med Clinical-proteomic classification and precision treatment strategy of chordoma |
