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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 67685-1-Ig (same clone as 67685-1-PBS)

    WB analysis using 67685-1-Ig (same clone as 67685-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67685-1-Ig (NF45 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 67685-1-Ig (same clone as 67685-1-PBS)

    IP experiment of HeLa using 67685-1-Ig (same clone as 67685-1-PBS)

    IP result of anti-NF45 (IP:67685-1-Ig, 4ug; Detection:67685-1-Ig 1:3000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67685-1-Ig (same clone as 67685-1-PBS)

    IF Staining of HepG2 using 67685-1-Ig (same clone as 67685-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NF45 antibody (67685-1-Ig, Clone: 1A3A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67685-1-Ig (same clone as 67685-1-PBS)

    IHC staining of human colon cancer using 67685-1-Ig (same clone as 67685-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67685-1-Ig (NF45 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67685-1-Ig (same clone as 67685-1-PBS)

    IHC staining of human breast cancer using 67685-1-Ig (same clone as 67685-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67685-1-Ig (NF45 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

NF45 Monoclonal antibody, PBS Only

NF45 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 67685-1-PBS

产品说明书

CloneNo. 1A3A2

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

ILF2, NF45, PRO3063

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67685-1-PBS targets NF45 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag30388

Product name: Recombinant human NF45 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-390 aa of BC000382

Sequence: MRGDRGRGRGGRFGSRGGPGGGFRPFVPHIPFDFYLCEMAFPRVKPAPDETSFSEALLKRNQDLAPNSAEQASILSLVTKINNVIDNLIVAPGTFEVQIEEVRQVGSYKKGTMTTGHNVADLVVILKILPTLEAVAALGNKVVESLRAQDPSEVLTMLTNETGFEISSSDATVKILITTVPPNLRKLDPELHLDIKVLQSALAAIRHARWFEENASQSTVKVLIRLLKDLRIRFPGFEPLTPWILDLLGHYAVMNNPTRQPLALNVAYRRCLQILAAGLFLPGSVGITDPCESGNFRVHTVMTLEQQDMVCYTAQTLVRILSHGGFRKILGQEGDASYLASEISTWDGVIVTPSEKAYEKPPEKKEGEEEEENTEEPPQGEEEESMETQE

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin enhancer binding factor 2, 45kDa
别名 ILF2, NF45, PRO3063
计算分子量 43 kDa
观测分子量40 kDa
GenBank蛋白编号BC000382
基因名称 ILF2
Gene ID (NCBI) 3608
RRIDAB_2882878
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ12905
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin-2 enhancer binding factor 2 (ILF2), also named as nuclear factor 45 (NF45), plays important roles in regulating interleukin-2 expression in mammals. It forms a heterodimeric complex with ILF3, which may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA [PMID:11739746,17129403].

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