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验证数据展示

  • WB analysis of HEK-293 using 66642-1-Ig (same clone as 66642-1-PBS)

    WB analysis of HEK-293 using 66642-1-Ig (same clone as 66642-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66642-1-Ig (NEUROG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66642-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 66642-1-Ig (same clone as 66642-1-PBS)

    IF Staining of rat brain using 66642-1-Ig (same clone as 66642-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NEUROG1 antibody (66642-1-Ig, Clone: 1E1H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66642-1-PBS in a different storage buffer formulation.

NEUROG1 Monoclonal antibody, PBS Only

NEUROG1 Monoclonal Antibody for WB, IF-P, ELISA
Cat No. 66642-1-PBS

产品说明书

CloneNo. 1E1H6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF-P, ELISA

别名

1E1H6, AKA, bHLHa6, Class A basic helix-loop-helix protein 6, Math4C

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66642-1-PBS targets NEUROG1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26438

Product name: Recombinant human NEUROG1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-237 aa of BC008687

Sequence: MPARLETCISDLDCASSSGSDLSGFLTDEEDCARLQQAASASGPPAPARRGAPNISRASEVPGAQDDEQERRRRRGRTRVRSEALLHSLRRSRRVKANDRERNRMHNLNAALDALRSVLPSFPDDTKLTKIETLRFAYNYIWALAETLRLADQGLPGGGARERLLPPQCVPCLPGPPSPASDAESWGSGAAAASPLSDPSSPAASEDFTYRPGDPVFSFPSLPKDLLHTTPCFIPYH

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 neurogenin 1
别名 1E1H6, AKA, bHLHa6, Class A basic helix-loop-helix protein 6, Math4C
计算分子量 237 aa, 26 kDa
观测分子量 20-25 kDa
GenBank蛋白编号BC008687
基因名称 NEUROG1
Gene ID (NCBI) 4762
RRIDAB_2882001
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ92886
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NEUROG1, also named BHLHA6, NEUROD3, NGN, and NGN1, is a 237 amino acid protein, which is expressed in embryonic nervous system. NEUROG1 acts as a transcriptional regulator that is involved in the initiation of neuronal differentiation. It activates transcription by binding to the E box (5'-CANNTG-3'). NEUROG1 can associate with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis.

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