验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, NCI-H1299 cells, U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55345-1-AP targets NEURL4 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | neuralized homolog 4 (Drosophila) |
| 别名 | Neuralized-like protein 4, Neuralized like protein 4, KIAA1787 |
| 计算分子量 | 167 kDa |
| 观测分子量 | 166 kDa |
| GenBank蛋白编号 | NM_032442 |
| 基因名称 | NEURL4 |
| Gene ID (NCBI) | 84461 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96JN8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NEURL4, also known as KIAA1787, has 6 highly conserved neuralized homology repeats (NHRs), which are thought to mediate protein-protein interactions. NEURL4 localizes to the daughter centriole and prevents formation of ectopic microtubule-organizing centers. NEURL4 promotes CCP110 ubiquitination and proteasome-dependent degradation, maintaining normal centrosomal homeostasis and preventing the formation of abnormal microtubule tissue centers (MTOCs) by countering CP110 accumulation(PMID: 22441691, 22261722).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NEURL4 antibody 55345-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |