- Featured Product
- KD/KO Validated
NEURL Polyclonal antibody
NEURL Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, mouse brain tissue, Y79 cells, SH-SY5Y cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
18898-1-AP targets NEURL in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IP, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | NEURL fusion protein Ag13531 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | neuralized homolog (Drosophila) |
| Synonyms | h neu, h neuralized 1, neu, neu 1, Neuralized like protein 1A, NEURL, NEURL1, NEURL1A, RING finger protein 67, RNF67 |
| Calculated Molecular Weight | 59 kDa |
| Observed Molecular Weight | 60-62 kDa |
| GenBank Accession Number | BC026336 |
| Gene Symbol | NEURL |
| Gene ID (NCBI) | 9148 |
| RRID | AB_10642557 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O76050 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NEURL(Neuralized-like protein 1A) is also named as NEURL1, NEURL1A, RNF67.Neurl1 is a RING-dependent E3 ubiquitin ligase that can regulate Notch signaling in mammals and it expression promotes lysosomal degradation of Jagged1 in cells and blocks signaling from Jagged1-expressing cells to neighboring Notch-expressing cells. Neurl-dependent turnover of Jagged1 and inhibition of Notch signaling depends upon N-terminal myristoylation and plasma membrane localization of Neurl1(PMID:18077452).This antibody is specific to NEURL.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NEURL antibody 18898-1-AP | Download protocol |
| IP protocol for NEURL antibody 18898-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest CB1 agonism prolongs therapeutic window for hormone replacement in ovariectomized mice.
|
