验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HL-60 cells, Jurkat cells, K-562 cells, THP-1 cells |
| Positive IHC detected in | mouse testis tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse small intestine tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27879-1-AP targets NEU3 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | Human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | NEU3 fusion protein Ag27259 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sialidase 3 (membrane sialidase) |
| 别名 | cDNA FLJ78026, NEU3, NEU3 protein, SIAL3 |
| 计算分子量 | 48 kDa |
| 观测分子量 | 48, 52 kDa |
| GenBank蛋白编号 | NM_006656.5 |
| 基因名称 | NEU3 |
| Gene ID (NCBI) | 10825 |
| RRID | AB_2881002 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UQ49 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NEU3 antibody 27879-1-AP | Download protocol |
| IHC protocol for NEU3 antibody 27879-1-AP | Download protocol |
| IF protocol for NEU3 antibody 27879-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
JCI Insight The mRNA binding protein DDX3 mediates TGF-β1 upregulation of translation and promotes pulmonary fibrosis | ||
Food Funct Polyphenol from millet bran increases the sensitivity of colorectal cancer cells to oxaliplatin by blocking the ganglioside GM3 catabolism.
| ||
Exp Lung Res Changes in lung sialidases in male and female mice after bleomycin aspiration | ||
