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验证数据展示

  • WB analysis of PC-12 using 67032-1-Ig (same clone as 67032-1-PBS)

    WB analysis of PC-12 using 67032-1-Ig (same clone as 67032-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67032-1-Ig (NEU1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67032-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 67032-1-Ig (same clone as 67032-1-PBS)

    IHC staining of human pancreas cancer using 67032-1-Ig (same clone as 67032-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67032-1-Ig (NEU1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67032-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 67032-1-Ig (same clone as 67032-1-PBS)

    IF Staining of U-251 using 67032-1-Ig (same clone as 67032-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using 67032-1-Ig (NEU1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67032-1-PBS in a different storage buffer formulation.

NEU1 Monoclonal antibody, PBS Only

NEU1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67032-1-PBS

产品说明书

CloneNo. 1F6D1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

N acetyl alpha neuraminidase 1, Lysosomal sialidase, G9 sialidase, EC:3.2.1.18, Acetylneuraminyl hydrolase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67032-1-PBS targets NEU1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 NEU1 fusion protein Ag27945 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 sialidase 1 (lysosomal sialidase)
别名 N acetyl alpha neuraminidase 1, Lysosomal sialidase, G9 sialidase, EC:3.2.1.18, Acetylneuraminyl hydrolase
计算分子量 45 kDa
观测分子量 45-48 kDa
GenBank蛋白编号BC011900
基因名称 NEU1
Gene ID (NCBI) 4758
RRIDAB_2882347
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99519
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NEU1 (lysosomal sialidase) encodes a kind of lysosomal enzyme that could cleave terminal silic acids from glycoproteins or glycolipids to regulate various biological processes by conformational change (PMID:28130415). In the lysosome, NEU1 is one member of a heterotrimeric complex, the others are beta-galactosidase and cathepsin A. The NEU1 is widely expressed in mammalian tissues and involved in lysosomal storage disorder sialidosis, autoimmune diseases and the malignancy and metastasis of cancer cells (PMID:19075514). NUE1 also expresses on the plasma membrane where it modulates several signalling molecules about inflamation, exocytosis, phagocytosis, cell adhesion and proliferation (PMID:21928149). Addition, it is reported that mutation in NEU1 of human could lead to sialidosis (PMID:14517945).