验证数据展示
经过测试的应用
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29936-1-AP targets NETO1 in IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag32177 Product name: Recombinant human NETO1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 23-213 aa of NM_138999 Sequence: TKKGTEKQTTSETQKSVQCGTWTKHAEGGIFTSPNYPSKYPPDRECIYIIEAAPRQCIELYFDEKYSIEPSWECKFDHIEVRDGPFGFSPIIGRFCGQQNPPVIKSSGRFLWIKFFADGELESMGFSARYNFTPDPDFKDLGALKPLPACEFEMGGSEGIVESIQIMKEGKATASEAVDCKWYIRAPPRSK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | neuropilin (NRP) and tolloid (TLL)-like 1 |
| 别名 | BTCL1, NETO 1 |
| 计算分子量 | 60 kDa |
| GenBank蛋白编号 | NM_138999 |
| 基因名称 | NETO1 |
| Gene ID (NCBI) | 81832 |
| RRID | AB_3742322 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TDF5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The NETO1 protein (neuropilin and tolloid-like protein 1) is a member of the NETO family. It is a type I transmembrane protein primarily expressed in the central nervous system. Serving as an important auxiliary subunit of glutamate receptors (especially kainate receptors and NMDA receptors), NETO1 plays a critical role in synaptic plasticity, learning, and memory by regulating receptor trafficking, gating properties, and synaptic localization. Its abnormalities are associated with neuropsychiatric disorders such as schizophrenia and epilepsy.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for NETO1 antibody 29936-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
