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验证数据展示

  • WB analysis using 81802-2-RR

    WB analysis using 81802-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81802-2-RR (NESP55,GNAS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 81802-2-RR

    WB analysis of MCF-7 using 81802-2-RR

    WB result of NESP55,GNAS antibody (81802-2-RR; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NESP55,GNAS transfected MCF-7 cells.

  • IF Staining of MCF-7 using 81802-2-RR

    IF Staining of MCF-7 using 81802-2-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NESP55,GNAS antibody (81802-2-RR, Clone: 251463D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 81802-2-RR

    Affinity and Kinetic Characterization of 81802-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81802-2-RR against Human NESP55,GNAS were performed. The affinity constant is below 1 pM.

  • KD/KO Validated

NESP55,GNAS Recombinant monoclonal antibody

NESP55,GNAS Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 81802-2-RR

产品说明书

CloneNo. 251463D5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

GNAS, GNAS1, GPIPIRRH peptide, LHAL tetrapeptide, NESP55

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSH-SY5Y cells, MCF-7 cells, Jurkat cells, MCGF-7 cells, mouse liver tissue, rat liver tissue
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy GNAS Ready-To-Use IHC Kit. GNAS primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81802-2-RR targets NESP55,GNAS in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0197

Product name: Recombinant human NESP55,GNAS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 230-394 aa of BC002722

Sequence: ERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GNAS complex locus
别名 GNAS, GNAS1, GPIPIRRH peptide, LHAL tetrapeptide, NESP55
计算分子量45 kDa
观测分子量46 kDa
GenBank蛋白编号BC002722
基因名称 GNAS
Gene ID (NCBI) 2778
RRIDAB_3743396
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP63092
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The near two bands represent two different isoforms. The expression profile/molecular weight observed are consistent with what has been described in the literature (PMID : 3258290).

实验方案

Product Specific Protocols
IF protocol for NESP55,GNAS antibody 81802-2-RRDownload protocol
WB protocol for NESP55,GNAS antibody 81802-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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