验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human brain tissue, HepG2 cells, LNCaP cells, A549 cells, HCT 116 cells, Jurkat cells, pig brain tissue |
| Positive IHC detected in | human cerebellum tissue, human colon tissue, human colon cancer tissue, human heart tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
60131-1-Ig targets Neudesin/NENF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, pig |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag8387 Product name: Recombinant human NENF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 33-172 aa of BC008823 Sequence: QTPRPAERGPPVRLFTEEELARYGGEEEDQPIYLAVKGVVFDVTSGKEFYGRGAPYNALTGKDSTRGVAKMSLDPADLTHDTTGLTAKELEALDEVFTKVYKAKYPIVGYTARRILNEDGSPNLDFKPEDQPHFDIKDEF 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | neuron derived neurotrophic factor |
| 别名 | NENF, Neudesin, 4G9E12, Cell immortalization-related protein 2, Neuron-derived neurotrophic factor |
| 计算分子量 | 172 aa, 19 kDa |
| 观测分子量 | 19 kDa, 16 kDa |
| GenBank蛋白编号 | BC008823 |
| 基因名称 | NENF |
| Gene ID (NCBI) | 29937 |
| RRID | AB_2150987 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UMX5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Neudesin neurotrophic factor (NENF, also known as CIR2, SPUF, and SCIRP10) acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival, which is localized to mitochondria and endoplasmic reticulum by PINK1 and PARK7 (PMID: 31536960). NENF in the adult brain is expected to play roles in the maintenance and protection of neurons in an autocrine/paracrine manner (PMID: 15605373). It greatly increases cAMP levels in neural precursor cells and might activate a Gs-protein-coupled receptor that could activate the MAPK, PKA, and PI-3K signal pathways (PMID: 16547973).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Neudesin/NENF antibody 60131-1-Ig | Download protocol |
| IHC protocol for Neudesin/NENF antibody 60131-1-Ig | Download protocol |
| WB protocol for Neudesin/NENF antibody 60131-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
