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验证数据展示

  • FC experiment of A431 using 84135-4-RR

    FC experiment of A431 using 84135-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug NELF-B Recombinant antibody (84135-4-RR, Clone:241434F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • WB analysis using 84135-4-RR

    WB analysis using 84135-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84135-4-RR (NELF-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 84135-4-RR

    IF Staining of A431 using 84135-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NELF-B antibody (84135-4-RR, Clone: 241434F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of BxPC-3 using 84135-4-RR

    FC experiment of BxPC-3 using 84135-4-RR

    1x10^6 BxPC-3 cells were intracellularly stained with 0.25 ug NELF-B Recombinant antibody (84135-4-RR, Clone:241434F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84135-4-RR

    Affinity and Kinetic Characterization of 84135-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84135-4-RR against Human NELF-B were performed. The affinity constant is 0.199 nM.

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NELFB Recombinant monoclonal antibody

NELFB Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84135-4-RR

产品说明书

CloneNo. 241434F12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

NELF B, Negative elongation factor B, KIAA1182, Cofactor of BRCA1, COBRA1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, BxPC-3 cells, A431 cells
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells, BxPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy NELFB Ready-To-Use IHC Kit. NELFB primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84135-4-RR targets NELFB in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag9599

Product name: Recombinant human NELF-B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 127-475 aa of BC011892

Sequence: VHYCTLRAELLMSLHDLDVGEICTVDPCHKFTWCLDACIRERFVDSKRARELQGFLDGVKKGQEQVLGDLSMILCDPFAINTLALSTVRHLQELVGQETLPRDSPDLLLLLRLLALGQGAWDMIDSQVFKEPKMEVELITRFLPMLMSFLVDDYTFNVDQKLPAEEKAPVSYPNTLPESFTKFLQEQRMACEVGLYYVLHITKQRNKNALLRLLPGLVETFGDLAFGDIFLHLLTGNLALLADEFALEDFCSSLFDGFFLTASPRKENVHRHALRLLIHLHPRVAPSKLEALQKALEPTGQSGEAVKELYSQLGEKLEQLDHRKPSPAQAAETPALELPLPSVPAPAPL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cofactor of BRCA1
别名 NELF B, Negative elongation factor B, KIAA1182, Cofactor of BRCA1, COBRA1
计算分子量580aa,66 kDa; 475aa,54 kDa
观测分子量 66 kDa
GenBank蛋白编号BC011892
基因名称 NELFB
Gene ID (NCBI) 25920
RRIDAB_3671694
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8WX92
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cofactor of BRCA1 (COBRA1) was first identified as a BRCA1-interacting protein and subsequently found to be the B subunit of the negative elongation factor complex (NELF-B). Cobra1 is essential for early embryogenesis (PMID: 19340312). The MW of this protein is 66 kDa, and this antibody specially recognises the 66 kDa protein.

实验方案

Product Specific Protocols
WB protocol for NELFB antibody 84135-4-RRDownload protocol
IF protocol for NELFB antibody 84135-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols