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验证数据展示

  • IHC staining of human colon cancer using 12244-1-AP

    IHC staining of human colon cancer using 12244-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12244-1-AP (NELF antibody) at dilution of 1:100 (under 10x lens).

  • WB analysis of HeLa using 12244-1-AP

    WB analysis of HeLa using 12244-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12244-1-AP (NELF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 12244-1-AP

    IF Staining of HeLa using 12244-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12244-1-AP (NELF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

NELF Polyclonal antibody

NELF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12244-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

NSMF, NMDA receptor synaptonuclear signaling and neuronal migration factor, Nasal embryonic luteinizing hormone-releasing hormone factor, Nasal embryonic LHRH factor

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, human brain tissue, human colon tissue, mouse testis tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12244-1-AP targets NELF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2888

Product name: Recombinant human NELF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-287 aa of BC016862

Sequence: ERKRRKRENDSASVIQRNFRKHLRMVGSRRVKAQTFAERRERSFSRSWSDPTPMKADTSHDSRDSSDLQSSHCTLDEAFEDLDWDTEKGLEAVACDTEGFVPPKVMLISSKVPKAEYIPTIIRRDDPSIIPILYDHEHATFEDILEEIERKLNVYHKGAKIWKMLIFCQGGPGHLYLLKNKVATFAKVEKEEDMIHFWKRLSRLMSKVNPEPNVIHIMGCYILGNPNGEKLFQNLRTLMTPYRVTFESPLELSAQGKQMIETYFDFRLYRLWKSRQHSKLLDFDDVL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nasal embryonic LHRH factor
别名 NSMF, NMDA receptor synaptonuclear signaling and neuronal migration factor, Nasal embryonic luteinizing hormone-releasing hormone factor, Nasal embryonic LHRH factor
计算分子量 507 aa, 56 kDa
观测分子量 54-60 kDa
GenBank蛋白编号BC016862
基因名称 NELF
Gene ID (NCBI) 26012
RRIDAB_2150819
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6X4W1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NSMF, also named as NELF (Nasal embryonic LHRH factor), couples NMDA-sensitive glutamate receptor signaling to the nucleus and triggers long-lasting changes in the cytoarchitecture of dendrites and spine synapse processes. NSMF has some isoforms with MW 44 kDa and 55-60 kDa. This antibody recognizes all the isoforms.

实验方案

Product Specific Protocols
IF protocol for NELF antibody 12244-1-APDownload protocol
IHC protocol for NELF antibody 12244-1-APDownload protocol
WB protocol for NELF antibody 12244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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