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验证数据展示

  • WB analysis using 87253-1-RR (same clone as 87253-1-PBS)

    WB analysis using 87253-1-RR (same clone as 87253-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87253-1-RR (NEK7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87253-1-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 87253-1-RR (same clone as 87253-1-PBS)

    FC experiment of A549 using 87253-1-RR (same clone as 87253-1-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug NEK7 Recombinant monoclonal antibody (87253-1-RR, Clone:252501C6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 87253-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 87253-1-RR (same clone as 87253-1-PBS)

    IHC staining of mouse spleen using 87253-1-RR (same clone as 87253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 87253-1-RR (NEK7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87253-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NEK7 antibody included
  • IHC staining of mouse spleen using 87253-1-RR (same clone as 87253-1-PBS)

    IHC staining of mouse spleen using 87253-1-RR (same clone as 87253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 87253-1-RR (NEK7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87253-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NEK7 antibody included
  • Affinity of 87253-1-RR

    Affinity of 87253-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87253-1-RR against Human NEK7 were performed. The affinity constant is 5.65 nM.

NEK7 Recombinant monoclonal antibody, PBS Only

NEK7 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 87253-1-PBS

产品说明书

CloneNo. 252501C6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

NimA related protein kinase 7

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NEK7 Ready-To-Use IHC Kit. NEK7 primary antibody included.

产品信息

87253-1-PBS targets NEK7 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag33629

Product name: Recombinant human NEK7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-36 aa of NM_133494

Sequence: MDEQSQGMQGPPVPQFQPQKALRPDMGYNTLANFRI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NIMA (never in mitosis gene a)-related kinase 7
别名 NimA related protein kinase 7
计算分子量35
观测分子量 32-35 kDa
GenBank蛋白编号NM_133494
基因名称 NEK7
Gene ID (NCBI) 140609
RRIDAB_3745433
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8TDX7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mammalian NIMA-related kinases (NEKs) represent a family of serine/threonine kinases, named NEK1-NEK11, which are implicated in the control of several aspects of mitosis and are involved in non-mitotic functions (PMID: 33364979). NIMA-related kinase 7 (NEK7) is the smallest NIMA-related kinase (NEK) in mammals. NEK7 is identified as a highly conserved serine or threonine kinase, which is not only crucial for mitosis entry, cell cycle progression, cell division, and mitotic process, but also expressed in brain, heart, lung, liver, spleen, and other issues (PMID: 31787755).

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