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验证数据展示

  • WB analysis using 31389-1-AP (same clone as 31389-1-PBS)

    WB analysis using 31389-1-AP (same clone as 31389-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31389-1-AP (NEK4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31389-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 31389-1-AP (same clone as 31389-1-PBS)

    IHC staining of mouse testis using 31389-1-AP (same clone as 31389-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31389-1-AP (NEK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31389-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NEK4 antibody included
  • IHC staining of mouse testis using 31389-1-AP (same clone as 31389-1-PBS)

    IHC staining of mouse testis using 31389-1-AP (same clone as 31389-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31389-1-AP (NEK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31389-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NEK4 antibody included

NEK4 Polyclonal antibody, PBS Only

NEK4 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 31389-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

STK2, pp12301, NRK2, NimA related protein kinase 4

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NEK4 Ready-To-Use IHC Kit. NEK4 primary antibody included.

产品信息

31389-1-PBS targets NEK4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag34929

Product name: Recombinant human NEK4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 429-779 aa of BC063044

Sequence: SSDIVTGEKNEPVKPLQPLIKEQKPKDQDQVAGECIIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVRRDLFAFQESPPRFLPSHPIVGKVDVTSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRPLSARERRRLKQSQEEMSSSGPSVRKASLSVAGPGKPQEEDQPLPARRLSSDCSVTQERKQIHCLSEDELSSSTSSTDKSDGDYGEGKGQTNEINALVQLMTQTLKLDSKESCEDVPVANPVSEFKLHRKYRDTLILHGKVAEEAEEIHFKELPSAIMPGSEKIRRLVEVLRTDVIRGLGVQLLEQVYDLLEEEDEFDREVSVSLTVSRCLCYR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NIMA (never in mitosis gene a)-related kinase 4
别名 STK2, pp12301, NRK2, NimA related protein kinase 4
计算分子量 781 aa, 88 kDa
观测分子量 95-100 kDa
GenBank蛋白编号BC063044
基因名称 NEK4
Gene ID (NCBI) 6787
RRIDAB_3669960
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP51957
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Neks (NIMA-related kinases) are a group of serine-threonine kinases that are related to NIMA, their ortholog from Aspergillus nidulans, which is essential for cells to enter in mitosis. NEK4 was initially identified as STK2, from serine/threonine kinase 2, in a study using a kinase specific cDNA library from human breast cancer tumors or breast cancer cells.

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